Download the full-sized PDF
Permanent link (DOI): https://doi.org/10.7939/R3M902G90
This file is in the following communities:
|Agricultural, Food and Nutritional Science, Department of|
This file is in the following collections:
|Journal Articles (Agricultural, Food and Nutritional Science)|
Differences in whole blood gene expression associated with infection time-course and extent of fetal mortality in a reproductive model of type 2 porcine reproductive and respiratory syndrome virus (PRRSV) infection Open Access
- Author or creator
Wilkinson, Jamie M.
Lunney, Joan K.
Harding, John C. S.
Plastow, Graham S.
- Additional contributors
Immune Receptor Signaling
- Type of item
- Journal Article (Published)
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection of pregnant females causes fetal death and increased piglet mortality, but there is substantial variation in the extent of reproductive pathology between individual dams. This study used RNA-sequencing to characterize the whole blood transcriptional response to type 2 PRRSV in pregnant gilts during the first week of infection (at 0, 2, and 6 days post-inoculation), and attempted to identify gene expression signatures associated with a low or high level of fetal mortality rates (LFM and HFM; n = 8/group) at necropsy, 21 days post-inoculation. The initial response to infection measured at 2 days post-inoculation saw an upregulation of genes involved in innate immunity, such as interferon-stimulated antiviral genes and inflammatory markers, and apoptosis. A concomitant decrease in expression of protein synthesis and T lymphocyte markers was observed. By day 6 the pattern had reversed, with a drop in innate immune signaling and an increase in the expression of genes involved in cell division and T cell signaling. Differentially expressed genes (DEGs) associated with extremes of litter mortality rate were identified at all three time-points. Among the 15 DEGs upregulated in LFM gilts on all three days were several genes involved in platelet function, including integrins ITGA2B and ITGB3, and the chemokine PF4 (CXCL4). LFM gilts exhibited a higher baseline expression of interferon-stimulated and pro-inflammatory genes prior to infection, and of T cell markers two days post-infection, indicative of a more rapid progression of the immune response to PRRSV. This study has increased our knowledge of the early response to PRRSV in the blood of pregnant gilts, and could ultimately lead to the development of a biomarker panel that can be used to predict PRRSV-associated reproductive pathology.
- Date created
- License information
- Attribution 4.0 International
- Citation for previous publication
Wilkinson, J. M., Ladinig, A., Bao, H., Kommadath, A., Stothard, P., Lunney, J. K., Harding, J. C. S., & Plastow, G. S. (2016). Differences in whole blood gene expression associated with infection time-course and extent of fetal mortality in a reproductive model of type 2 porcine reproductive and respiratory syndrome virus (PRRSV) infection. PLoS ONE, 11(4), e0153615 [21 pages]. http://dx.doi.org/10.1371/journal.pone.0153615
- Link to related item
- Date Uploaded
- Date Modified
- Audit Status
- Audits have not yet been run on this file.
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 2156604
Last modified: 2017:09:06 16:37:22-06:00
Original checksum: acfff56483842228810db0c1b7955a6f
Well formed: true
Status message: Invalid destination object offset=2009234
File title: Differences in Whole Blood Gene Expression Associated with Infection Time-Course and Extent of Fetal Mortality in a Reproductive Model of Type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Infection
File author: Jamie M. Wilkinson, Andrea Ladinig, Hua Bao, Arun Kommadath, Paul Stothard, Joan K. Lunney, John C. S. Harding, Graham S. Plastow
Page count: 21