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Permanent link (DOI): https://doi.org/10.7939/R31Z41Z66

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Non-enzymatic browning in glucosamine and glucosamine-peptides reaction systems as a source of antioxidant and flavouring compounds Open Access

Descriptions

Other title
Subject/Keyword
Glucosamine
Non-enzymatic browning
Maillard reaction
Caramelization
Antioxidant activity
Taste enhancing activity
Alpha-dicarbonyl
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Hong, Pui Khoon
Supervisor and department
Betti, Mirko (Food Science)
Examining committee member and department
Gaenzle, Michael (Food Science)
Chen, Lingyun (Food Science)
Farmer, Anna (Human Nutrition)
Ahn, Dong Uk (Animal Science, Iowa State University)
Department
Department of Agricultural, Food, and Nutritional Science
Specialization
Food Science and Technology
Date accepted
2016-06-10T15:12:19Z
Graduation date
2016-06:Fall 2016
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
Non-enzymatic browning is a common and complex reaction that occurs in everyday cooking—indeed, it is a crucial step in food processing. A typical browning process includes both the Maillard reaction and caramelization, which normally occur only at extreme temperatures to produce both desired and distinctive flavours and an intense brown colour in foods. On one hand, advanced stage Maillard reaction compounds can be toxic, such as acrylamide and 5-hydroxymethylfurfural (5-HMF). And yet on the other hand, Maillard reaction compounds can possess bioactivities including taste enhancing, antioxidant and antimicrobial capacities; collectively these are known as Maillard reacted peptides (MRPs). This bizarre paradox among non-enzymatic browning reaction compounds has long deserved a more in depth investigation under controlled conditions. To achieve these positive bioactivities yet reduce the accumulation of the harmful compounds associated with the Maillard reaction, a research strategy was proposed to produce and understand MRPs at lower temperatures. However, information on the antioxidant and sensorial properties MRPs at moderate temperatures is scarce. Glucosamine (GlcN) is an amino sugar recently revealed to be capable of triggering a fast Maillard reaction with protein at 25˚C. Additionally, due to the presence of both a carbonyl and amino group within the same molecule, GlcN can form substantial dicarbonyls at 37˚C—the precursors to desirable flavours. The main objective of this thesis was to study taste enhancement and antioxidant activity of GlcN-peptides in GlcN model systems. A total of 3 studies were designed representing the main building blocks of this project. The first study focused on the potential of GlcN to modifying protein hydrolysates at 25 and 37˚C in a fish gelatin hydrolysates-GlcN model. Modification of the hydrolysates by GlcN was accomplished by two approaches: firstly, a Maillard-based glycation with GlcN, and secondly, an enzymatic glycosylation catalyzed by the transglutaminase (TGase), condensing the primary amine group of GlcN with the carboxamide group of a glutamine residue in a peptide. GlcN-induced modification was achieved at both 25 and 37˚C, and the antioxidant and antimicrobial activities were improved compared to native hydrolysates. The second study focused on the taste enhancing property of GlcN-modified hydrolysed meat proteins produced at 37 and 50˚C in the presence or absence of TGase. Samples were formulated into seasoning compositions and evaluated by untrained consumers. The meat protein hydrolysate was perceived as the saltiest (p<0.05) whereas the glycated hydrolysate produced at 50˚C tended (p= 0.0593) to be the most savoury seasoning composition. This further confirmed the role of GlcN as an important component in modified hydrolysate by eliciting an umami taste, despite its inability to strike a balance between eliciting saltiness and savouriness. The non-enzymatic browning of GlcN was further investigated in the third study. Chemical-physico changes and antioxidant activity were monitored in 3-level factorial models: in phosphate buffer versus ammonium hydroxide solution, at 40 versus 60˚C, and incubated up to 48 h. Incubation at 40˚C for 6 h produced a yellow-coloured caramel with the greatest levels of anti-radical activity and diacetyl—a volatile flavour compound of dicarbonyls. Overall, this thesis showed GlcN to be a reactive amino sugar capable of key rapid peptide reactions and self-modifications at moderate temperatures. Compounds from these GlcN-mediated non-enzymatic browning reactions not only represent important flavour and colour agents, but also showed promise as antimicrobials and antioxidants. A future paradigm shift is anticipated for GlcN, evolving from its current status as a health supplement to become a multi-functional food ingredient.
Language
English
DOI
doi:10.7939/R31Z41Z66
Rights
This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for the purpose of private, scholarly or scientific research. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
Citation for previous publication
Hong, P.K., Gottardi, D., Ndagijimana, M., and Betti, M. 2014. Glycation and transglutaminase mediated glycosylation of fish gelatin peptides with glucosamine enhance bioactivity. Food Chem 142(1):258-293.Hong, P.K., Ndagijimana, M., and Betti, M. 2016. Glucosamine-induced glycation of hydrolysed meat proteins in the presence or absence of transglutaminase: Chemical modifications and taste-enhancing activity. Food Chem 197(B):1143-1152.Hong, P.K., and Betti, M. 2016. Non-enzymatic browning reaction of glucosamine at mild conditions: Relationship between colour formation, radical scavenging activity and α-dicarbonyl compounds production. Food Chem 212: 234-243.

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