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Permanent link (DOI): https://doi.org/10.7939/R3125QM3Z

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Studies on the Binding Specificity of Intelectin Open Access

Descriptions

Other title
Subject/Keyword
Galactofuranose
Surface Plasmon Resonance
Intelectin
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Khalil, Amira, IA
Supervisor and department
Lowary, Todd
Examining committee member and department
Vocadlo, David (Chemistry, Simon Fraser University)
Campbell, Robert (Chemistry U of A)
West, Fredrick (Chemistry U of A)
Cairo, Christopher (Chemistry U of A)
Lowary, Todd (Chemistry U of A)
Department
Department of Chemistry
Specialization

Date accepted
2014-11-26T11:05:09Z
Graduation date
2015-06
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
Our primary goal of this study was to obtain a better understanding for the carbohydrates binding interactions with human intelectin (hIntL). Studying the binding specificity of hIntL could help in discovering its natural substrate and might give us a clue about its role in the innate immune response as well as its pathophysiological action in various diseases. In this project, recombinant expression of intelectins was successfully done using HeLa cells and H5V cells. Yeast (Pachia pastoris) did not proove to be a promising expression system for hIntL. Using chemical synthesis, compounds (3.1–3.9) were obtained to be screened as ligands for hIntL. Herein, we used surface plasmon resonance (SPR) as our analytical tool to study hIntL binding specificity towards various synthesized Galf-containing oligosaccharides. Staudinger ligation chemistry as well as amine coupling were used to immobilize the carbohydrate epitopes to the SPR biosensor surface. Both hIntL-1 and hIntL-2 were screened against the derivatized surface.
Language
English
DOI
doi:10.7939/R3125QM3Z
Rights
This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for the purpose of private, scholarly or scientific research. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
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