Studies on the Binding Specificity of Intelectin

• Author / Creator

  Khalil, Amira, IA

• Our primary goal of this study was to obtain a better understanding for the carbohydrates binding interactions with human intelectin (hIntL). Studying the binding specificity of hIntL could help in discovering its natural substrate and might give us a clue about its role in the innate immune response as well as its pathophysiological action in various diseases. In this project, recombinant expression of intelectins was successfully done using HeLa cells and H5V cells. Yeast (Pachia pastoris) did not proove to be a promising expression system for hIntL. Using chemical synthesis, compounds (3.1–3.9) were obtained to be screened as ligands for hIntL. Herein, we used surface plasmon resonance (SPR) as our analytical tool to study hIntL binding specificity towards various synthesized Galf-containing oligosaccharides. Staudinger ligation chemistry as well as amine coupling were used to immobilize the carbohydrate epitopes to the SPR biosensor surface. Both hIntL-1 and hIntL-2 were screened against the derivatized surface.

• Subjects / Keywords

  • Intelectin
  • Galactofuranose
  • Surface Plasmon Resonance

• Graduation date

  Spring 2015

• Type of Item

  Thesis

• Degree

  Doctor of Philosophy

• DOI

  https://doi.org/10.7939/R3125QM3Z

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.