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Permanent link (DOI): https://doi.org/10.7939/R3H98ZS8D

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Differential expression of miRNAs in Brassica napus root following infection with Plasmodiophora brassicae Open Access

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Author or creator
Verma, Shiv
Rahman, Muhammad H.
Deyholos, Michael K.
Basu, Urmila
Kav, Nat N. V.
Additional contributors
Subject/Keyword
Auxins
MicroRNAs
Lateral Roots
Arabidopsis Thaliana
Microarrays
Developmental Signaling
Gene Expression
Plant Hormones
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Canola (oilseed rape, Brassica napus L.) is susceptible to infection by the biotrophic protist Plasmodiophora brassicae, the causal agent of clubroot. To understand the roles of microRNAs (miRNAs) during the post-transcriptional regulation of disease initiation and progression, we have characterized the changes in miRNA expression profiles in canola roots during clubroot disease development and have compared these to uninfected roots. Two different stages of clubroot development were targeted in this miRNA profiling study: an early time of 10-dpi for disease initiation and a later 20-dpi, by which time the pathogen had colonized the roots (as evident by visible gall formation and histological observations). P. brassicae responsive miRNAs were identified and validated by qRT-PCR of miRNAs and the subsequent validation of the target mRNAs through starBase degradome analysis, and through 5′ RLM-RACE. This study identifies putative miRNA-regulated genes with roles during clubroot disease initiation and development. Putative target genes identified in this study included: transcription factors (TFs), hormone-related genes, as well as genes associated with plant stress response regulation such as cytokinin, auxin/ethylene response elements. The results of our study may assist in elucidating the role of miRNAs in post-transcriptional regulation of target genes during disease development and may contribute to the development of strategies to engineer durable resistance to this important phytopathogen.
Date created
2014
DOI
doi:10.7939/R3H98ZS8D
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Attribution 4.0 International
Citation for previous publication
Verma, S., Rahman, M. H., Deyholos, M. K., Basu, U., & Kav, N. N. V. (2014). Differential expression of miRNAs in Brassica napus root following infection with Plasmodiophora brassicae. PLoS ONE, 9(1), e86648 [11pages].  http://dx.doi.org/10.1371/journal.pone.0086648

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