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Permanent link (DOI): https://doi.org/10.7939/R3QV3CH5T

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The LmSNF1 gene is required for pathogenicity in the canola blackleg pathogen Leptosphaeria maculans Open Access

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Author or creator
Feng, Jie
Zhang, Hui
Strelkov, Stephen E.
Hwang, Sheau-Fang
Additional contributors
Subject/Keyword
Fungi
Pathogenesis
Glucose
Pectins
Fungal Genomics
Fungal Genetics
Gene Knockout
Plant Fungal Pathogens
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Leptosphaeria maculans is a fungal pathogen causing blackleg in canola. Its virulence has been attributed, among other factors, to the activity of hydrolytic cell wall degrading enzymes (CWDEs). Studies on the pathogenicity function of CWDEs in plant pathogenic fungi have been difficult due to gene redundancy. In microorganisms many CWDE genes are repressed by glucose and derepressed by the function of the sucrose non-fermenting protein kinase 1 gene (SNF1). To address the molecular function of SNF1 in L. maculans, the ortholog of SNF1 (LmSNF1) was cloned and functionally characterized using a gene knockout strategy. Growth of the LmSNF1 knockout strains was severely disrupted, as was sporulation, spore germination and the ability to attach on the plant surface. When inoculated on canola cotyledons, the LmSNF1 knockout strains could not cause any symptoms, indicating the loss of pathogenicity. The expression of 11 selected CWDE genes and a pathogenicity gene (LopB) was significantly down-regulated in the LmSNF1 knockout strains. In conclusion, knockout of LmSNF1 prevents L. maculans from properly derepressing the production of CWDEs, compromises the utilization of certain carbon sources, and impairs fungal pathogenicity on canola.
Date created
2014
DOI
doi:10.7939/R3QV3CH5T
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Attribution 4.0 International
Citation for previous publication
Feng, J., Zhang, H., Strelkov, S. E., & Hwang, S. F. (2014). The LmSNF1 gene is required for pathogenicity in the canola blackleg pathogen Leptosphaeria maculans. PLoS ONE, 9(3), e92503 [11 pages].  http://dx.doi.org/10.1371/journal.pone.0092503

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