ERA

Download the full-sized PDF of Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seedsDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3736MF9Z

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Agricultural, Food and Nutritional Science, Department of

Collections

This file is in the following collections:

Journal Articles (Agricultural, Food and Nutritional Science)

Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds Open Access

Descriptions

Author or creator
Wickramarathna, Aruna D.
Siloto, Rodrigo M. P.
Mietkiewska, Elzbieta
Singer, Stacy D.
Pan, Xue
Weselake, Randall J.
Additional contributors
Subject/Keyword
AtROD1
Linum usitatissimum
α-linolenic Acid
PUFA
Saccharomyces cerevisiae
Phosphatidylcholine-diacyglycerol Interconversion
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Background Flax (Linum usitatissimum L.) is an agriculturally important crop with seed oil enriched in α-linolenic acid (18:3 cisΔ9, 12, 15; ALA). This polyunsaturated fatty acid (PUFA) is the major determinant for the quality of flax seed oil in food, nutraceuticals and industrial applications. The recently identified enzyme: phosphatidylcholine diacylglycerol cholinephosphotransferase (PDCT), catalyzes the interconversion between phosphatidylcholine (PC) and diacylglycerol (DAG), and has been shown to play an important role in PUFA accumulation in Arabidopsis thaliana seeds. Methods Two flax PDCT genes were identified using homology-based approach. Results In this study, we describe the isolation and characterization of two PDCT genes from flax (LuPDCT1 and LuPDCT2) with very high nucleotide sequence identity (97%) whose deduced amino acid sequences exhibited approximately 55% identity with that of A. thaliana PDCT (AtROD1). The genes encoded functionally active enzymes that were strongly expressed in developing embryos. Complementation studies with the A. thaliana rod1 mutant demonstrated that the flax PDCTs were capable of restoring PUFA levels in planta. Furthermore, PUFA levels increased in Saccharomyces cerevisiae when the flax PDCTs were co-expressed with FATTY ACID DESATURASES (FADs), FAD2 and FAD3, while seed-specific expression of LuPDCT1 and LuPDCT2 in A. thaliana resulted in 16.4% and 19.7% increases in C18-PUFAs, respectively, with a concomitant decrease in the proportion of oleic acid (18:1 cisΔ9; OA). Conclusions The two novel PDCT homologs from flax are capable of increasing C18-PUFA levels substantially in metabolically engineered yeast and transgenic A. thaliana seeds. These flax PDCT proteins appear to play an important dual role in the determination of PUFA content by efficiently channelling monounsaturated FAs into PC for desaturation and moving the resulting PUFAs out of PC for subsequent use in TAG synthesis. These results indicate that flax PDCTs would be useful for bioengineering of oil crops to increase PUFA levels for applications in human food and nutritional supplements, animal feed and industrial bioproducts.
Date created
2015
DOI
doi:10.7939/R3736MF9Z
License information

Rights
Attribution 4.0 International
Citation for previous publication
Wickramarathna, A. D., Siloto, R. M. P., Mietkiewska, E., Singer, S. D., Pan, X., & Weselake, R. J. (2015). Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds. BMC Biotechnology, 15(63), [15 pages].  http://dx.doi.org/10.1186/s12896-015-0156-6

Source

Link to related item

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 1451869
Last modified: 2017:09:06 16:06:47-06:00
Filename: BMCBiotech_15_63.pdf
Original checksum: b1e960538c2124c550214e652d62fdf8
Well formed: true
Valid: false
Status message: Missing expected element in page number dictionary offset=829574
File title: Abstract
File title: Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds
File author: Aruna D Wickramarathna
Page count: 15
File language: EN
Activity of users you follow
User Activity Date