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Permanent link (DOI): https://doi.org/10.7939/R3RJ49661

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Development of imaging-based techniques to examine signalling properties of channel catfish (Ictalurus punctatus) leukocyte immune-type receptors (IpLITRs) Open Access

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Other title
Subject/Keyword
phagocytosis
flow cytometry
imaging
leukocyte immune-type receptors
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Jones, Lena O.
Supervisor and department
Stafford, James (Biological Sciences)
Examining committee member and department
Magor, Katharine (Biological Sciences)
McKenzie, Debbie (Biological Sciences)
Touret, Nicolas (Biochemistry)
Stafford, James (Biological Sciences)
Department
Department of Biological Sciences
Specialization
Physiology, Cell, and Developmental Biology
Date accepted
2017-02-07T11:36:20Z
Graduation date
2017-06:Spring 2017
Degree
Master of Science
Degree level
Master's
Abstract
Cells of the innate immune system have the ability to rapidly detect and eliminate pathogens using surface-expressed immunoregulatory receptors that translate extracellular binding into effector functions such as degranulation, cytokine secretion, cell-mediated cytotoxicity, and phagocytosis. Teleost leukocyte immune-type receptors (LITRs) are a family of immunoregulatory receptors capable of inducing effector responses when heterologously expressed in mammalian immune cells. The mechanisms by which these receptors induce effector responses remain unknown; however, these effector responses are all dependent on cytoskeletal rearrangements. Many of the intracellular signaling components that facilitate cytoskeleton remodelling are conserved through evolution due to the ubiquitous requirement for cells to change shape or move. The main objective of my thesis was to develop imaging-based assays to examine LITR-induced signaling activities that culminate in effector responses such as phagocytosis. Using the phagocytic process as a bioassay, I developed experimental strategies employing cellular transfections, imaging flow cytometry, and confocal microscopy to monitor the LITR-mediated phagocytic process and associated signaling. I showed that two LITR-types induced very distinct cytoskeletal remodelling patterns to induce a phagocytic response. Additionally, cytoskeletal remodelling was mirrored by the distribution of a phospholipid species known to participate in the induction of filamentous actin (the main cytoskeletal component) polymerization. Overall, my work set the stage for testing potential signaling activities that IpLITRs are believed to induce.
Language
English
DOI
doi:10.7939/R3RJ49661
Rights
This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for the purpose of private, scholarly or scientific research. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
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