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Permanent link (DOI): https://doi.org/10.7939/R3KS6JF94

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Quantitative trait loci and genomewide association mapping in western Canadian spring wheat (Triticum aestivum L.) Open Access

Descriptions

Other title
Subject/Keyword
genomewide association mapping
quantitative trait loci
early maturity
wheat
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Chen, Hua
Supervisor and department
Spaner, Dean (Agricultural, Food, and Nutritional Science)
Examining committee member and department
Ayele, Belay (University of Manitoba)
Rahman, Habibur (Agricultural, Food, and Nutritional Science)
Yang, Rong-Cai (Agricultural, Food, and Nutritional Science)
Strelkov, Stephen (Agricultural, Food, and Nutritional Science)
Department
Department of Agricultural, Food, and Nutritional Science
Specialization
Plant Science
Date accepted
2016-01-06T15:24:08Z
Graduation date
2016-06
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
Early maturity, grain yield and grain protein content are some of the important traits in western Canadian wheat breeding programs. A series of experiments were conducted to explore the genetic basis of days to heading, and maturity, plant height, grain protein, grain yield and related traits. In a spring wheat population of 187 recombinant inbred lines genotyped with 341 Diversity Array Technology (DArT) polymorphic markers, a total of 21 quantitative trait loci (QTLs) were identified for all phenotypic traits recorded, except plant height and grain protein content. Two earliness per se QTLs were mapped on chromosomes 1A (QEps.dms-1A) and 4A (QEps.dms-4A) in all three growing seasons, contributing 15-27% and 8-10%, respectively, to the total genetic variation in days to maturity. The two earliness QTLs and Vrn-B1 exhibited additive interaction. In the same population, lines carrying the resistant allele of Lr34/Yr18 were taller, matured earlier, yielded less grain with lower test weights than lines without Lr34/Yr18. Lines with Lr34/Yr18 also exhibited lower leaf and stripe rust infection than lines with the susceptible allele. The failure to combine Lr34/Yr18 with high yield, protein, and SDS sedimentation suggested single seed descent or doubled haploid populations for the combined selection of multiple quantitatively inherited traits, and simply one molecular marker, would require population sizes in excess of at least 500 to have any possibility of selection success. Genetic diversity analysis for earliness related and plant height reducing genes in 82 spring wheat cultivars registered in western Canada through eight diagnostic DNA markers suggested breeding efforts in western Canada have resulted in the incorporation of vernalization and photoperiod insensitive and height reducing genes in modern cultivars to promote early maturity, to make use of off-season nurseries in other parts of the world and to improve lodging tolerance. Using genome-wide association mapping (GWAS). we identified a total of 152 significant marker-trait associations; however, there were only 18 genomic regions that consisted of clusters of 3 to 20 significant single nucleotide polymorphisms (SNPs) across 12 chromosomes, including two regions each for grain yield, test weight and protein content, six regions for plant height and six other coincident regions that were associated with two or three traits. The genomic region associated with plant height on chromosome 4B showed high linkage disequilibrium (r2 > 0.80) with the semi-dwarfing gene Rht-B1. Results of these studies suggest that besides the widely used semi-dwarf and early maturity related genes, there is a wide spectrum of loci available that could be used for modulating plant height, days to maturity, grain yield and grain protein content in western Canadian wheat germplasm.
Language
English
DOI
doi:10.7939/R3KS6JF94
Rights
This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for the purpose of private, scholarly or scientific research. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
Citation for previous publication
Chen H, Iqbal M, Perez-Lara E, Yang R-C, Pozniak C, Spaner D (2015) Earliness per se quantitative trait loci and their interaction with Vrn-B1 locus in a spring wheat population. Molecular Breeding 35:1-14.Chen, H., Moakhar, N. P., Iqbal, M., Pozniak, C., Hucl, P., & Spaner, D. Genetic variation for flowering time and height reducing genes and important traits in western Canadian spring wheat. Euphytica, 1-14.

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