ERA

Download the full-sized PDF of Genome-wide whole blood microRNAome and transcriptome analyses revealed miRNA-mRNA regulated host response to foodborne pathogen Salmonella infection in swineDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3TH8BR4F

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Roy Berg Kinsella Research Ranch

Collections

This file is in the following collections:

Journal Articles (Kinsella Ranch)

Genome-wide whole blood microRNAome and transcriptome analyses revealed miRNA-mRNA regulated host response to foodborne pathogen Salmonella infection in swine Open Access

Descriptions

Author or creator
Bao, Hua
Kommadath, Arun
Liang, Guanxiang
Sun, Xu
Arantes, Adriano S.
Tuggle, Christopher K.
Bearson, Shawn M.D.
Plastow, Graham S.
Stothard, Paul
Guan, Le L.
Additional contributors
Subject/Keyword
Regulatory Networks
Systems Biology
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
To understand the role of miRNAs in regulating genes involved in host response to bacterial infection and shedding of foodborne pathogens, a systematic profiling of miRNAs and mRNAs from the whole blood of pigs upon Salmonella challenge was performed. A total of 62 miRNAs were differentially expressed post infection (false discovery rate <0.1). An integrative analysis of both the differentially expressed miRNAs and mRNAs using sequence-based miRNA target prediction and negative correlation of miRNA-mRNA profiles helped identify miRNA-mRNA networks that may potentially regulate host response to Salmonella infection. From these networks, miR-214 and miR-331-3p were identified as new candidates potentially associated with Salmonella infection. An miRNA seed sequence analysis suggested that these miRNAs regulate several critical immune-related genes including SLC11A1, PIGE-108A11.3 and VAV2. We showed that challenged pigs had reduced miR-214 expression and increased miR-331-3p expression in the whole blood. Furthermore, the expression of the proposed targets of miR-214 (SLC11A1 and PIGE-108A11.3) increased while that of the proposed target of miR-331-3p (VAV2) decreased following challenge (expression changes confirmed by in vitro assays). Based on these observations, we propose potential roles for miR-214 and miR-331-3p in regulation of immune responses to Salmonella infection.
Date created
2015/07/31
DOI
doi:10.7939/R3TH8BR4F
License information
Attribution 4.0 International
Rights

Citation for previous publication
Bao, H. et al. Genome-wide whole blood microRNAome and transcriptome analyses reveal miRNA-mRNA regulated host response to foodborne pathogen Salmonella infection in swine. Sci. Rep. 5, 12620; doi: 10.1038/srep12620 (2015).
Source

Link to related item

File Details

Date Uploaded
Date Modified
2016-06-07T15:48:23.576+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 1560370
Last modified: 2016:06:07 09:48:30-06:00
Filename: SR_5_12620_1.pdf
Original checksum: 7079a044949230f4d0bb11465cd078b3
Copyright note: © 2015 Nature Publishing Group
File title: Genome-wide whole blood microRNAome and transcriptome analyses reveal miRNA-mRNA regulated host response to foodborne pathogen Salmonella infection in swine
File author: Hua Bao
Page count: 12
Activity of users you follow
User Activity Date