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Permanent link (DOI): https://doi.org/10.7939/R3QX46

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Theses and Dissertations

Role of triacylglycerol hydrolase in hepatic lipid droplet metabolism Open Access

Descriptions

Other title
Subject/Keyword
mouse hepatocytes
lipid droplet
very-low density lipoprotein
apolipoprotein E
triacylglycerol hydrolase
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Wang, Huajin
Supervisor and department
Lehner, Richard (Pediatrics and Cell Biology)
Examining committee member and department
Brasaemle, Dawn (Nutritional Sciences, Rutgers University)
Simmen, Thomas (Cell Biology)
Vance, Dennis (Biochemistry)
Hobman, Tom (Cell Biology)
Department
Department of Cell Biology
Specialization

Date accepted
2009-10-02T20:57:13Z
Graduation date
2009-11
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
The majority of triacylglycerol (TG) utilized for very-low density lipoproteins (VLDL) assembly is derived from lipolysis-reesterification of TG stored in lipid droplets (LDs). At least two types of LDs exist in hepatocytes – cytosolic LDs (CLDs) and the microsomal-associated luminal LDs (LLDs). An endoplasmic reticulum luminal lipase, triacylglycerol hydrolase (TGH), was shown to participate in mobilizing intracellular TG stored in LDs. However, it is not clear which pool of TG is hydrolyzed by TGH. LLDs are present in the same subcellular compartment as TGH and thus may serve as the substrate pool for this enzyme. Results presented in this thesis describe the isolation and characterization of LLDs from mouse liver microsomes, providing the first biochemical evidence for the presence of these LDs. LLDs differ from CLDs or VLDL particles in both protein and lipid compositions. TGH was found to associate with LLDs, suggesting it may hydrolyze this pool of TG. Other VLDL secretion related proteins were also found on LLDs, including apolipoprotein E and microsomal triglyceride transfer protein. It was determined that LLDs constitute a minor pool of intracellular TG. Thus, quantitatively TG stored in CLDs may provide the major TG source for VLDL assembly. Overexpression of TGH resulted in increased mobilization of not only LLDs but also CLDs. The mechanism by which TGH may regulate the metabolism of CLDs was investigated in wild type and TGH-deficient mouse hepatocytes. It was found that TGH deficiency led to morphological changes in CLDs and diminished the rate at which preformed CLDs obtain newly formed TG (CLD growth). An alternative mechanism for CLD growth was also explored by tracing the incorporation of fluorescent fatty acids analogues into CLDs by live-cell imaging. The results suggested that newly synthesized TG are assembled into LDs through TGH-dependent and TGH-independent mechanisms. The role of apoE as cofactor for TGH mediated lipolysis was investigated. Obtained results suggested that the absence of apoE in hepatocytes compromised the lipolysis/reesterification process mediated by TGH. Future studies in this direction should further explore the interaction of apoE with TGH and whether the interaction affects the stability of TGH at the lipid-water interface.
Language
English
DOI
doi:10.7939/R3QX46
Rights
License granted by Huajin Wang (huajin@ualberta.ca) on 2009-10-02T05:17:20Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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