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Permanent link (DOI): https://doi.org/10.7939/R3RN30B6R

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Comparative transcriptomic analysis of rectal tissue from beef steers revealed reduced host immunity in Escherichia coli O157:H7 super-shedders Open Access

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Author or creator
Wang, Ou
Liang, Guanxiang
McAllister, Tim A.
Plastow, Graham
Stanford, Kim
Selinger, Brent
Guan, Le L.
Additional contributors
Subject/Keyword
Gene Expression
Cattle
T Cells
B Cells
Transcriptome Analysis
MicroRNAs
Lymphocytes
RNA Sequencing
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Super-shedder cattle are a major disseminator of E. coli O157:H7 into the environment, and the terminal rectum has been proposed as the primary E. coli O157:H7 colonization site. This study aimed to identify host factors that are associated with the super-shedding process by comparing transcriptomic profiles in rectal tissue collected from 5 super-shedder cattle and 4 non-shedder cattle using RNA-Seq. In total, 17,859 ± 354 genes and 399 ± 16 miRNAs were detected, and 11,773 genes were expressed in all animals. Fifty-eight differentially expressed (DE) genes (false discovery rate < 0.05) including 11 up-regulated and 47 down-regulated (log 2 (fold change) ranged from -5.5 to 4.2), and 2 up-regulated DE miRNAs (log 2 (fold change) = 2.1 and 2.5, respectively) were identified in super-shedders compared to non-shedders. Functional analysis of DE genes revealed that 31 down-regulated genes were potentially associated with reduced innate and adaptive immune functions in super-shedders, including 13 lymphocytes membrane receptors, 3 transcription factors and 5 cytokines, suggesting the decreased key host immune functions in the rectal tissue of super-shedders, including decreased quantity and migration of immune cells such as lymphocytes, neutrophils and dendritic cells. The up-regulation of bta-miR-29d-3p and the down regulation of its predicted target gene, regulator of G-protein signaling 13, suggested a potential regulatory role of this miRNA in decreased migration of lymphocytes in super-shedders. Based on these findings, the rectal tissue of super-shedders may inherently exhibit less effective innate and adaptive immune protection. Further study is required to confirm if such effect on host immunity is due to the nature of the host itself or due to actions mediated by E. coli O157:H7.
Date created
2016/03/09
DOI
doi:10.7939/R3RN30B6R
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Attribution 4.0 International
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Citation for previous publication
Wang, O., Liang, G., McAllister, T.A., Plastow, G., Stanford, K., Selinger, B., Guan, L.L. (2016) Comparative transcriptomic analysis of rectal tissue from beef steers revealed reduced host immunity in Escherichia coli O157:H7 super-shedders. PLoS ONE 11(3). doi: 10.1371/journal.pone.0151284
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File title: Comparative Transcriptomic Analysis of Rectal Tissue from Beef Steers Revealed Reduced Host Immunity in Escherichia coli O157:H7 Super-Shedders
File author: Ou Wang, Guanxiang Liang, Tim A. McAllister, Graham Plastow, Kim Stanford, Brent Selinger, Le Luo Guan
Page count: 19
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