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Permanent link (DOI): https://doi.org/10.7939/R3D93T

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Investigating the clavam gene cluster in Streptomyces antibioticus Tü1718 Open Access

Descriptions

Other title
Subject/Keyword
clavam
gene clusters
Streptomyces antibioticus Tü1718
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Goomeshi Nobary, Sarah
Supervisor and department
Jensen, Susan (Biological Sciences)
Examining committee member and department
Leskiw, Brenda (Biological Sciences)
Feldman, Mario (Biological Sciences)
Jensen, Susan (Biological Sciences)
Clive, Derrick (Chemistry)
Department
Department of Biological Sciences
Specialization
Microbiology and Biotechnology
Date accepted
2012-05-30T15:41:19Z
Graduation date
2011-06
Degree
Master of Science
Degree level
Master's
Abstract
Production of clavams (including clavulanic acid, a potent β-lactamase inhibitor) has been studied in Streptomyces clavuligerus. All clavams share the early biosynthetic steps. However, many of the late steps of the pathway remain unclear. Streptomyces antibioticus Tü1718 was chosen for this study because it produces two 5S clavams (different from clavulanic acid in their stereochemistry) and no clavulanic acid. After screening a cosmid library of the gDNA of this bacterium for a cas gene (encoding an early biosynthetic enzymes), a cosmid was selected. After finding additional early genes (bls, pah, ceaS, oat) and some putative late pathway genes (an aminotransferase and ORFs similar to c7p, cvm1 and orfA of S. clavuligerus) in the regions flanking cas, the whole cosmid was sequenced. Several novel genes were encountered including ntr that showed similarity to a nitroreductase. Knock out mutation of the cas and ntr gene abolished clavam production in S. antibioticus Tü1718.
Language
English
DOI
doi:10.7939/R3D93T
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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