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Permanent link (DOI): https://doi.org/10.7939/R3HQ3S68Z

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A novel group of zebrafish Class II ADP-ribosylation factors and their potential role in photoreceptor development Open Access

Descriptions

Other title
Subject/Keyword
Zebrafish
ADP-ribosylation Factors
Arf4
Arfs,
photoreceptor
BFA
morpholino
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Sharifi, Navid
Supervisor and department
Melançon, Paul (Cell Biology)
Examining committee member and department
Allison, Ted (Biological Sciences)
Dacks, Joel (Cell Biology)
Department
Department of Cell Biology
Specialization

Date accepted
2013-10-04T08:47:42Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
In this study we identified an unknown set of Class II Arf isoforms in zebrafish. Bioinformatics analyses grouped these paralogues into three distinct clades. Using antisense morpholino gene knockdown, we tested the effect of loss of each zebrafish Class II Arf on early development and photoreceptor organization. At 5 days post fertilization gross morphological defects in Arf II-A2 morphants was evident, while photoreceptor disorganization was seen in Arf II-C1 morphants. In situ hybridization experiments confirmed Arf II-C1 expression in regions of the brain and in the retina. We also found that zebrafish Arf II-C1 does not possess properties that are conserved in other Arfs. Most Class II Arfs examined in this study from different species localized to ERGIC-53 positive puncta in COS1 cells; while Arf II-C1 did not. Furthermore, in contrast to hArf4, Arf II-C1 quickly became cytosolic and dissociated from punctate structures in the presence of BFA.
Language
English
DOI
doi:10.7939/R3HQ3S68Z
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File size: 16722578
Last modified: 2015:10:12 15:51:13-06:00
Filename: Sharifi_Navid_Fall 2013.pdf
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File title: Microsoft Word - Sharifi_Navid_Fall 2013.docx
File author: Navid Sharifi
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