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Permanent link (DOI): https://doi.org/10.7939/R3SS32

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Introgression of Blackleg Resistance into Brassica napus from Brassica carinata Open Access

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Other title
Subject/Keyword
Introgression, blackleg, ovule culture, backcross
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Rahman, Md Mostafizur
Supervisor and department
Rahman, Habibur (Agricultural, Food and Nutritional Science)
Examining committee member and department
Kutcher, H. R. (Agriculture and Food and Nutritional Science)
Yeh, C. Francis (Renewable Resources)
Strelkov, Stephen (Agricultural, Food and Nutritional Science)
Department
Department of Agricultural, Food, and Nutritional Science
Specialization

Date accepted
2011-11-16T18:41:34Z
Graduation date
2012-06
Degree
Master of Science
Degree level
Master's
Abstract
Blackleg, caused by Leptosphaeria maculans, is one of the most damaging diseases of oilseed rape, Brassica napus. Interspecific hybridization between B. napus and B. carinata was done to transfer resistance to PG4 type blackleg pathotype from B. carinata into B. napus. In vitro ovule culture and in vivo seed set techniques were applied for the production of interspecific hybrids, where ovule culture was more efficient than in vivo seed set; and ovule culture in NN liquid medium was more efficient than B5 solid medium. All the interspecific F1 hybrids were resistant to blackleg. The F1 hybrids were recurrently backcrossed to B. napus and selection for cotyledon and adult plant resistance performed in each generation. In the backcross generations, significant number of seedlings with cotyledon resistance was found to be susceptible at the adult plant stage suggesting that cotyledon and adult plant resistance is under different genetic control in B. carinata. The proportion of resistant plants decreased with the progression of backcrossing- apparently due to loss of B. carinata chromosome(s) carrying the resistance.
Language
English
DOI
doi:10.7939/R3SS32
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File author: Rahman, MD Mostafizur
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