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Introgression of Blackleg Resistance into Brassica napus from Brassica carinata Open Access


Other title
Introgression, blackleg, ovule culture, backcross
Type of item
Degree grantor
University of Alberta
Author or creator
Rahman, Md Mostafizur
Supervisor and department
Rahman, Habibur (Agricultural, Food and Nutritional Science)
Examining committee member and department
Kutcher, H. R. (Agriculture and Food and Nutritional Science)
Yeh, C. Francis (Renewable Resources)
Strelkov, Stephen (Agricultural, Food and Nutritional Science)
Department of Agricultural, Food, and Nutritional Science

Date accepted
Graduation date
Master of Science
Degree level
Blackleg, caused by Leptosphaeria maculans, is one of the most damaging diseases of oilseed rape, Brassica napus. Interspecific hybridization between B. napus and B. carinata was done to transfer resistance to PG4 type blackleg pathotype from B. carinata into B. napus. In vitro ovule culture and in vivo seed set techniques were applied for the production of interspecific hybrids, where ovule culture was more efficient than in vivo seed set; and ovule culture in NN liquid medium was more efficient than B5 solid medium. All the interspecific F1 hybrids were resistant to blackleg. The F1 hybrids were recurrently backcrossed to B. napus and selection for cotyledon and adult plant resistance performed in each generation. In the backcross generations, significant number of seedlings with cotyledon resistance was found to be susceptible at the adult plant stage suggesting that cotyledon and adult plant resistance is under different genetic control in B. carinata. The proportion of resistant plants decreased with the progression of backcrossing- apparently due to loss of B. carinata chromosome(s) carrying the resistance.
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