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Permanent link (DOI): https://doi.org/10.7939/R3D21RS5G

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THE APPLICATION OF ALGAE FOR THE REMOVAL OF METALS AND NAPHTHENIC ACIDS FROM OIL SANDS TAILINGS POND WATER Open Access

Descriptions

Other title
Subject/Keyword
Parachlorella kessleri
Oil sands tailings pond water
algae
metal removal
algae-bacteria consortium
naphthenic acid removal
treatment
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Mahdavi, Hamed
Supervisor and department
Yang Liu, Civil and Environmental Engineering Department
Ania C. Ulrich, Civil and Environmental Engineering Department
Examining committee member and department
Ian Buchanan, Civil and Environmental Engineering Department, University of Alberta
Leonidas Perez-Estrada, Civil and Environmental Engineering Department, University of Alberta
Angus Chu, Engineering for the Environment Program at the University of Calgary
Department
Department of Civil and Environmental Engineering
Specialization
Environmental Engineering
Date accepted
2014-07-09T13:17:43Z
Graduation date
2014-11
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
Bitumen recovery produces a large volume of TPW, which must be reclaimed using an efficient and cost-effective method. In this thesis, the ability of algae for in situ treatment of TPW, with a focus on metal and total acid-extractable organics (TAOs) removal, was assessed. An indigenous alga, found in cyclone overflow water, was capable of removing metals 53Cr, Mn, Co, 60Ni, 65Cu, 66Zn, As, 88Sr, 95Mo and Ba. Through the amplification of the 23S rRNA gene, the indigenous alga was identified as Parachlorella kessleri. Highest metal removal was achieved with the highest nutrient supplements (1.98 mM NO3- and 0.20 mM PO43-) in Syncrude TPW, and the lowest nutrient supplements (0.24 mM NO3- and 0.016 mM PO43-) in Albian TPW. This implies that higher concentrations of nutrient supplements do not necessarily improve the metal removal efficiency. FT-IR spectra revealed the presence of amide I, amide II bands, and carboxylic functional groups on the surface of P. kessleri; these sites likely contribute to metal removal. However, an acid-base titration showed that the carboxylic group was the only active proton binding site for metal binding. Intracellular bioaccumulation was the dominant mechanism of metal removal, with extracellular bioaccumulation and precipitation playing a smaller role. For the biodegradation of TAOs, a consortium of indigenous algae-bacteria was employed. Bacteria demonstrated the greatest removal of TAOs with a half-life removal rate of 203 days (first-order kinetics). The TAO removal rate did not correlate with detoxification of TPW, where most toxicity reduction was observed in samples containing the algae-bacteria consortium. Principal component analysis (PCA) was conducted on the FT-IR spectra. The significant loading wavenumbers, which likely indicated bio-transformed functional groups and bonds in the TAO molecules, were identified as: hydroxyl, carbocyclic and amid groups along with C-H, aryl-H, aryl-OH and N-H bonds. The observations from this research indicate that indigenous algae play an important role in the removal of metals and TAOs from oil sands TPW. However, for future engineering application, more investigates are required to optimize the operating conditions to improve the removal efficiency.
Language
English
DOI
doi:10.7939/R3D21RS5G
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Mahdavi, H., Ulrich, A.C., Liu, Y., 2012. “Metal removal from oil sands tailings pond water by indigenous micro-alga.” Chemosphere. 89, 350-354Mahdavi, H., Liu, Y., and Ulrich, A. C. (2013). “Partitioning and Bioaccumulation of Metals from Oil Sands Process Affected Water in Indigenous Parachlorella Kessleri”, Chemosphere, 90(6), 1893-1899.

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