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Permanent link (DOI): https://doi.org/10.7939/R3M06Q

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Electrophysiological Properties of a Quail Neuroretina Cell Line (QNR/D): Effects of Growth Hormone? Open Access

Descriptions

Other title
Subject/Keyword
Growth Hormone
Retinal Ganglion Cell
Calcium Channels
Retina
Ion Channels
RGC-5
QNR/D
Potassium Channels
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Andres, Alexis D
Supervisor and department
Harvey, Stephen (Physiology)
Examining committee member and department
Sanders, Esmond (Physiology)
Karpinski, Edward (Physiology)
Kline, Loren (Physiology)
Milner, Rachel (Biochemistry)
Department
Department of Physiology
Specialization

Date accepted
2012-08-07T15:52:13Z
Graduation date
2012-11
Degree
Master of Science
Degree level
Master's
Abstract
The expression of retinal ganglion cell protein markers and voltage-gated ion channels in QNR/D cells was investigated using immunocytochemistry and the whole cell patch clamp technique, respectively. Additionally, since these cells express growth hormone and growth hormone receptor, the effects of exogenous growth hormone treatment on ion channel expression were also investigated. It was determined that QNR/D cells express retinal ganglion cell markers Islet-1 homeobox transcription factor, R4A antigenicity, neurofilament proteins as well as growth hormone and growth hormone receptor. It was also determined that these cells express vimentin filaments which are expressed in vivo in retinal ganglion cell precursors. The expression of a variety of voltage-gated currents was observed. These currents include T-type Ca2+ currents, and A-type and delayed rectifier K+ currents. Exogenous growth hormone treatment did not significantly alter the density of these currents or induce the expression of any other current types after 72 hours of exposure.
Language
English
DOI
doi:10.7939/R3M06Q
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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