ERA

Download the full-sized PDF of Characterization of Cox15p, a cytochrome c oxidase assembly factor and component of the eukaryotic heme A synthaseDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3GF0N70P

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

Characterization of Cox15p, a cytochrome c oxidase assembly factor and component of the eukaryotic heme A synthase Open Access

Descriptions

Other title
Subject/Keyword
heme A
Cox15p
cytochrome c oxidase
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Rumley, Alina C.
Supervisor and department
Glerum, Moira (Cell Biology)
Examining committee member and department
Hughes, Sarah (Medical Genetics)
Eitzen, Gary (Cell Biology)
Nargang, Frank (Biological Sciences)
Department
Medical Sciences-Medical Genetics
Specialization

Date accepted
2012-05-31T15:29:34Z
Graduation date
2011-11
Degree
Master of Science
Degree level
Master's
Abstract
Cytochrome c oxidase (COX) converts oxygen to water as part of oxidative phosphorylation. Studies in yeast estimate that more than forty different genes are required for COX assembly. The heme A prosthetic groups are essential for COX function and defects in heme A synthesis have been shown to underlie human COX deficiencies. The nuclear-encoded Cox15p has been proposed to have a role in heme A synthesis. I have characterized S. cerevisiae cox15 mutant strains with regards to respiratory growth, COX assembly, heme A levels, and stability of the mutant Cox15p. I have identified mutants with a novel phenotype. Initial studies suggest that a cox15 null strain has abnormal mitochondrial morphology and that Cox15p has a role in peroxide metabolism. My results further support the functioning of Cox15p in heme A biosynthesis and provide insight into the variable clinical phenotypes seen in patients with COX15 mutations.
Language
English
DOI
doi:10.7939/R3GF0N70P
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2014-04-25T00:40:00.737+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 26853123
Last modified: 2015:10:12 20:06:11-06:00
Filename: Rumley_Alina_Fall 2011.pdf
Original checksum: 61342ee2f49ccfa41cded39d8a585378
Well formed: true
Valid: true
Status message: File header gives version as 1.4, but catalog dictionary gives version as 1.3
File title: Thesis 5.A
File author: Andrea Pellegrino
Page count: 150
Activity of users you follow
User Activity Date