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Characterization of Cox15p, a cytochrome c oxidase assembly factor and component of the eukaryotic heme A synthase Open Access


Other title
heme A
cytochrome c oxidase
Type of item
Degree grantor
University of Alberta
Author or creator
Rumley, Alina C.
Supervisor and department
Glerum, Moira (Cell Biology)
Examining committee member and department
Hughes, Sarah (Medical Genetics)
Eitzen, Gary (Cell Biology)
Nargang, Frank (Biological Sciences)
Medical Sciences-Medical Genetics

Date accepted
Graduation date
Master of Science
Degree level
Cytochrome c oxidase (COX) converts oxygen to water as part of oxidative phosphorylation. Studies in yeast estimate that more than forty different genes are required for COX assembly. The heme A prosthetic groups are essential for COX function and defects in heme A synthesis have been shown to underlie human COX deficiencies. The nuclear-encoded Cox15p has been proposed to have a role in heme A synthesis. I have characterized S. cerevisiae cox15 mutant strains with regards to respiratory growth, COX assembly, heme A levels, and stability of the mutant Cox15p. I have identified mutants with a novel phenotype. Initial studies suggest that a cox15 null strain has abnormal mitochondrial morphology and that Cox15p has a role in peroxide metabolism. My results further support the functioning of Cox15p in heme A biosynthesis and provide insight into the variable clinical phenotypes seen in patients with COX15 mutations.
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