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Permanent link (DOI): https://doi.org/10.7939/R33X1G

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Comparative proteomic and metabolomic analysis of a Staphylococcus variant (SG1) cultured in the presence and absence of butanol Open Access

Descriptions

Other title
Subject/Keyword
quantitative metabolomics
butanol
quantitative proteomics
solvent tolerance
Staphylococcus warneri SG1
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Fu, Feifei
Supervisor and department
Weiner, Joel (Department of Biochemistry)
Li, Liang (Department of Chemistry)
Examining committee member and department
Le, Chris (Department of Laboratory Medicine and Pathology)
Weiner, Joel (Department of Biochemistry)
Li, Liang (Department of Chemistry)
Department
Department of Chemistry
Specialization

Date accepted
2013-08-27T11:49:57Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
My research focuses on proteomic and metabolomic analysis of a solvent tolerant strain of Staphylococcus warneri called SG1 cultured in the presence and absence of 1-Butanol. On the proteomic analysis, the tryptic digests of SG1 were either directly analyzed by two-dimensional liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry (2D-LC-ESI-QTOF MS) for protein identification or isotope labeled using dimethylation after guanidination (2-MEGA) followed by 2D-LC-ESI-QTOF MS for relative protein quantification. On the metabolomic analysis, the extracted metabolites were either labeled with 12C/13C dansyl chloride for relative quantitation of amine- and phenol-containing metabolites or labeled with 12C/13C p-dimethylaminophenacyl (DmPA) bromide for relative quantitation of carboxylic acid-containing metabolites. Finally, proteomic data and metabolomic data were combined and compared to help elucidate the solvent tolerant mechanism of SG1. The thesis work highlights the potential and significance of combining proteomic and metabolomic analyses for studying biological systems.
Language
English
DOI
doi:10.7939/R33X1G
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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