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Identifying and Characterizing Red Blood Cell Microvesiculation, Phosphatidylserine and CD47 Expression As a Predictor of Red Blood Cell In Vitro Quality Following Hypothermic Storage Open Access


Other title
Flow cytometry
Red Blood Cell
Hypothermic Storage
Phosphatidylserine and CD47 Expression
Red Blood Cell Microvesiculation
Type of item
Degree grantor
University of Alberta
Author or creator
Almizraq, Ruqayyah J
Supervisor and department
Acker, Jason (Supervisor, Department of Laboratory Medicine and Pathology)
Holovati, Jelena (Co-supervisor, Department of Laboratory Medicine and Pathology)
Examining committee member and department
Onell, Rodrigo (Department of Laboratory Medicine and Pathology)
Lew, Kristi (Department of Laboratory Medicine and Pathology)
Medical Sciences- Laboratory Medicine and Pathology

Date accepted
Graduation date
Master of Science
Degree level
Abstract: Proper preservation of the quality of stored red blood cells (RBC) is fundamental for successful blood transfusion. RBC microparticles (RMPs) have been shown to be harmful effectors of inflammation and coagulation posttransfusion, as well as in vitro indicators of RBC function and integrity throughout ex vivo storage. The main objectives of this thesis were to develop a flow cytometric analysis method to identify, characterize and quantify RMPs during hypothermic storage (HS) along with examining the effects of biochemical rejuvenation on RBC microvesiculation and membrane composition. This thesis demonstrated that HS conditions induce RBC microvesiculation and changes in CD47 and PS. This study also showed evidence that ATP restoration following the rejuvenation had no significant effect on RMPs generation or PS and CD47 expression throughout HS. The work presented in this thesis has furthered the field of the biopreservation by outlining techniques to improve blood transfusion.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Almizraq R, Tchir JDR, Holovati JL, Acker JP. Storage of red blood cells affects membrane composition, microvesiculation, and in vitro quality. Transfusion 2013. doi: 10.1111/trf.12080. [Epub ahead of print].Almizraq R, Bicalho B, Tchir JDR, Acker JP, Holovati JL. Assessment of the effects of rejuvenation on red blood cell membrane lipids, microvesiculation, phosphatidylserine and CD47 expression during hypothermic storage. Transfusion 2013.Jayme D.R Tchir, Jason P. Acker, Jelena L. Holovati. Rejuvenation of ATP during storage does not reverse effects of the hypothermic storage lesion. Transfusion 2013.

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