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Permanent link (DOI): https://doi.org/10.7939/R34C7J

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Development of an enantioselective two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method for the analysis of methylsulfonyl polychlorinated biphenyls in tissue extracts Open Access

Descriptions

Other title
Subject/Keyword
chiral two dimensional liquid chromatography
PCB metabolite
atmospheric pressure photoionization
tandem mass spectrometry
methylsulfonyl polychlorinated biphenyl
Greenland sledge dog
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Cooper, Victoria Irene
Supervisor and department
Li, Liang (Chemistry)
Wong, Charles (Chemistry)
Examining committee member and department
Li, Xing-Fang (Laboratory Medicine and Pathology)
Li, Liang (Chemistry)
Wong, Charles (Chemistry)
Department
Department of Chemistry
Specialization

Date accepted
2011-01-11T15:48:27Z
Graduation date
2011-06
Degree
Master of Science
Degree level
Master's
Abstract
An enantioselective heart-cut two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method was developed for the analysis of 25 methylsulfonyl polychlorinated biphenyl metabolites in tissue extracts. Enantioseparation was achieved for 9 out of the 10 chiral analytes in less than 91 minutes, improving upon previous gas chromatography-based methods. Use of a pyrenyl-ethyl silica column in the first dimension enabled separation of all but two pairs of isobaric analytes. Limits of detection of 0.01 to 1.73 ng on-column were achieved. The precision and accuracy were within acceptable limits, but poor sensitivity was achieved for several meta-methylsulfonyl-substituted congeners. Despite this limitation, the method was successfully applied to the analysis of Greenland sledge dog (Canis familiaris) plasma and adipose tissue extracts. Concentration and enantiomer fraction data are presented. None of the target analytes were detected in Norwegian glaucous gull (Larus hyperboreus) plasma extracts.
Language
English
DOI
doi:10.7939/R34C7J
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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