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Permanent link (DOI): https://doi.org/10.7939/R3W422

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Novel Mediators and Medical Prevention of Bladder Fibrosis After Partial Bladder Outlet Obstruction Open Access

Descriptions

Other title
Subject/Keyword
SLRP
Tadalafil
Oxybutynin
Bladder Outlet Obstruction
Bladder
Decorin
Biglycan
Rapamyin
Fibrosis
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Maciejewski, Conrad C
Supervisor and department
Metcalfe, Peter (Surgery)
Tredget, Edward (Surgery)
Examining committee member and department
Luyckx, Valerie (Medicine)
Ding, Jie (Surgery)
Metcalfe, Peter (Surgery)
Moore, Ronald (Surgery)
Tredget, Edward (Surgery)
Department
Department of Surgery
Specialization
Experimental Surgery
Date accepted
2012-08-24T09:48:14Z
Graduation date
2012-11
Degree
Master of Science
Degree level
Master's
Abstract
Introduction: Partial bladder outlet obstruction (pBOO) is a significant problem that can affect renal health. We hypothesize that changes occur in small leucine-rich proteoglycan (SLRP) composition of extracellular matrix over time in this process. We hypothesize that medical treatment of this condition will improve functional and molecular outcomes. Methods: Rats underwent surgical pBOO for 4-16 weeks time, and were compared to shams and untreated control animals. Urodynamic measurements were taken, bladders were weighed, measured for thickness, and frozen for RT-PCR and mass spectrometry. Results: Decorin and biglycan were found to be inversely related over the course of pBOO, with decorin decreasing in RNA and protein expression, and biglycan increasing. Treatment of animals with oxybutynin or tadalafil resulted in favorable urodynamic, histologic and molecular changes. Conclusion: SLRPs are differentially regulated in pBOO. Treatment with oxybutynin or tadalafil delayed progression of pBOO pathology. We believe that the process of pBOO is medically alterable.
Language
English
DOI
doi:10.7939/R3W422
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Differential Expression of Class I Small Leucine-Rich Proteoglycans in an Animal Model of Partial Bladder Outlet Obstruction. Maciejewski CC, Honardoust D, Tredget EE, Metcalfe PD. Journal of Urology, AAP Supplement 2012.

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Last modified: 2015:10:12 16:45:23-06:00
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File title: Compiled Thesis
File author: Conrad Maciejewski
Page count: 147
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