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Permanent link (DOI): https://doi.org/10.7939/R32J68B40

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The effects of fructose feeding on the quantal catecholamine release from adrenal chromaffin cells Open Access

Descriptions

Other title
Subject/Keyword
Adrenal chromaffin cells
Amperometry
Fructose-fed rats
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Simpson, Michael Robert
Supervisor and department
Dr. Fred Tse (Pharmacology/ Neuroscience)
Examining committee member and department
Dr. Matthias Braun (Pharmacology)
Dr. Glen Baker (Psychiatry/ Neuroscience)
Dr. Peter Smith (Pharmacology/ Neuroscience)
Department
Centre for Neuroscience
Specialization

Date accepted
2014-01-24T15:49:18Z
Graduation date
2014-06
Degree
Master of Science
Degree level
Master's
Abstract
The fructose-fed rat is an animal model of metabolic syndrome, which includes the development of hypertension. In other animal models of hypertension, an increase in the amount catecholamine released from chromaffin cells was reported. Employing carbon fibre amperometry, I tested the hypothesis that fructose feeding causes an increase in the amount of catecholamine released from individual adrenal chromaffin cells. When I stimulated chromaffin cells from fructose-fed rats with a non-selective acetylcholine agonist, they secreted more catecholamine than cells from age-matched control rats, although the amplitude of the rise in intracellular Ca2+ (measured by fura-2) was not increased. Fructose feeding also increased the amount of catecholamine released from individual secretory granules. These changes in catecholamine release were not reproduced when the cells were stimulated by elevating the extracellular concentration of K+. My findings suggest that fructose feeding may alter the expression or function of muscarinic acetylcholine receptors in chromaffin cells.
Language
English
DOI
doi:10.7939/R32J68B40
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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