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Permanent link (DOI): https://doi.org/10.7939/R3WH2DP37

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DESULFURIZATION OF OXIDIZED BITUMEN USING MICROORGANISMS THROUGH PROCESS-BASED DIRECTED EVOLUTION Open Access

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Other title
Subject/Keyword
Sequential Transfers
Self-Cycling Fermentation (SCF)
Biodesulfurization
Directed Evolution
Rhodococcus
Dibenzothiophene Sulfone (DBTO2)
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
De Man, Allison B
Supervisor and department
de Klerk, Arno (Chemical and Materials Engineering)
Sauvageau, Dominic (Chemical and Materials Engineering)
Examining committee member and department
Sauvageau, Dominic (Chemical and Materials Engineering)
Ulrich, Ania (Civil and Environmental Engineering)
de Klerk, Arno (Chemical and Materials Engineering)
Department
Department of Chemical and Materials Engineering
Specialization
Chemical Engineering
Date accepted
2014-01-03T15:28:35Z
Graduation date
2014-06
Degree
Master of Science
Degree level
Master's
Abstract
Many Rhodococcus species are capable of utilizing the 4S biodesulfurization pathway to convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2-HBP) and sulfite, the latter of which is partially incorporated into cell biomass. The first steps of the 4S pathway involve the oxidation of DBT into DBTO2 for further conversion. It is hypothesized that using DBTO2 as the direct substrate for desulfurization would result in faster growth rates and increased desulfurization activity. Process-based directed evolution strategies were performed, using DBTO2 as a selective pressure, with three Rhodococcus species to adapt the cultures and select for mutants with increased desulfurization activity. Process-based directed evolution was performed through two strategies: (i) sequential transfers and (ii) self-cycling fermentation (SCF). A change in desulfurization activity for Rhodococcus rhodochrous IGTS8 was observed and indicated a change in sulfur management in the cell. Rhodococcus erythropolis EPWF developed a gain-of-function mutation that enabled it to desulfurize DBTO2.
Language
English
DOI
doi:10.7939/R3WH2DP37
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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Last modified: 2015:10:12 16:05:32-06:00
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File title: Chapter 3: Research topic A
File author: Allison DeMan
Page count: 120
File language: en-US
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