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Permanent link (DOI): https://doi.org/10.7939/R3ZW6S

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Retinal Growth Hormone: An Autocrine/paracrine in the Developing Chick Retina Open Access

Descriptions

Other title
Subject/Keyword
growth hormone (GH)
autocrine
insulin-like growth factor-1 (IGF-1)
cell apoptosis
paracrine
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Lin, Wan-Ying
Supervisor and department
Steve Harvey (Physiology)
Examining committee member and department
Hamid R Habibi Bio Sci/U of C)
Ed Karpinski (Physiology)
Yves Sauve (Ophthalmology)
Esmond J. Sanders (Physiology)
Department
Department of Physiology
Specialization

Date accepted
2011-04-15T20:53:13Z
Graduation date
2011-06
Degree
Master of Science
Degree level
Master's
Abstract
The developing chick retina is an extrapituitary site of growth hormone (GH) synthesis and action. GH, GH receptor (GHR) and their mRNAs are present in the neural retina when the neural cells are undergoing proliferation and differentiation during early embryogenesis. It is thus likely that GH acts as an autocrine or paracrine in this location. The present study shows that intra-vitreal injection of a chick GH (cGH) small interfering RNA (siRNA) into the eyes of early embryos [embryonic day (ED) 4] suppresses GH expression in the neural retina and increases the incidence of spontaneous retinal cell death. Our current work also demonstrates a reduction of local IGF-1 expression after retinal GH gene knockdown, suggesting that GH action in retinal cells is regulated through IGF-1 signalling. These results demonstrate that retinal GH is an autocrine/paracrine hormone that acts as a neuroprotective factor in the retina of chick embryos.
Language
English
DOI
doi:10.7939/R3ZW6S
Rights
License granted by Wan-Ying Lin (wanying2@ualberta.ca) on 2011-04-15T19:07:17Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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