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Examination of the signalling properties and ligand-binding potential of stimulatory leukocyte immune-type receptors (IpLITRs) in the channel catfish (Ictalurus punctatus) Open Access


Other title
channel catfish
leukocyte immune-type receptors
ligand-binding potential
signalling properties
immune receptor
Type of item
Degree grantor
University of Alberta
Author or creator
Mewes-Ares, Jacqueline
Supervisor and department
Dr. James Stafford, Biological Sciences
Examining committee member and department
Dr. Luis Schang, Biochemistry
Dr. Andrew Keddie, Biological Sciences
Department of Biological Sciences

Date accepted
Graduation date
Master of Science
Degree level
Channel Catfish (Ictalurus punctatus, Rafinesque, 1818) leukocyte immune-type receptors (IpLITRs) are a family of proteins sharing structural and phylogenetic relationships with mammalian immune receptors. Based on their predicted signalling potential and ligand-binding properties, IpLITRs may be important in the control of immune cell effector responses in fish. The main objectives of this thesis were to determine how stimulatory IpLITRs activate cells and to develop assays for the screening of IpLITR ligands. Using cellular transfections, coimmunoprecipitation, and flow cytometry, I determined that stimulatory IpLITRs associate with specific adaptor molecules, which is required for their surface expression and signalling ability. These adaptors assemble with IpLITRs via their charged transmembrane regions and contain cytoplasmic tails encoding tyrosines that may initiate kinase pathways leading to immune cell activation. This study represents the first step towards elucidating how IpLITRs ‘turn on’ immune cells. Combined with the development of assays to identify IpLITR ligands, my work sets the stage for further investigations into the functional characterization of these receptors.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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