Download the full-sized PDF of Extraction of Triticale Distillers Grain Proteins for Adhesive DevelopmentDownload the full-sized PDF



Permanent link (DOI):


Export to: EndNote  |  Zotero  |  Mendeley


This file is in the following communities:

Graduate Studies and Research, Faculty of


This file is in the following collections:

Theses and Dissertations

Extraction of Triticale Distillers Grain Proteins for Adhesive Development Open Access


Other title
Distillers grain
Triticale protein
Protein modification
Protein extraction
Protein based adhesives
Type of item
Degree grantor
University of Alberta
Author or creator
Bandara, Nandika Priyantha
Supervisor and department
Dr. Jianping Wu, Department of Agricultural, Food and Nutritional Science
Examining committee member and department
Dr. Lingyun Chen, Department of Agricultural, Food and Nutritional Science
Dr. Hongbo Zeng, Department of Chemical and Materials Engineering
Department of Agricultural, Food and Nutritional Science

Date accepted
Graduation date
Master of Science
Degree level
Triticale (×Triticosecale Wittmackk) is being actively explored as a feedstock for bioethanol production in Canada. As the main co-product of bioethanol processing, triticale distillers grain contains 20-43% protein (dry basis), and mainly used as animal feed. The purpose of this study was to find new uses of triticale protein as adhesive. Proteins from triticale distillers wet grains (DWG) and distillers dried grains with solubles (DDGS) were extracted by five methods: pH shifting method, 60% ethanol, alkaline ethanol, glacial acetic acid, and enzyme-aided extraction. Extraction with alkaline ethanol and glacial acetic acid gave higher protein contents (~61–65%) than those obtained from other extraction methods (~23–24%); however, enzyme-aided extraction using Protex 6L yielded 75–82% protein at a content of 43–57%. To prepare adhesives, proteins were modified by NaOH, urea and glutaraldehyde; effects of modifications on protein structure were analyzed by FTIR, and their adhesion properties were measured by automated bonding evaluation system (ABES II). The highest (p<0.05) adhesion strength was observed in acetic acid extracted proteins; glutaraldehyde modification acetic acid extracted proteins increased the adhesion strength from 2.56, 0.84, 1.11 MPa to 3.86, 2.03, 2.60 MPa for dry, wet and soaked adhesion strength, respectively. Increases in α-helical conformation and molecular weight were observed for glutaraldehyde modified proteins. Development of adhesive from triticale proteins might represent new uses of triticale distillers grain.
License granted by Nandika Priyantha Bandara ( on 2011-09-28T16:51:01Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 1450059
Last modified: 2015:10:18 01:41:21-06:00
Filename: Bandara_Nandika_Fall_2011.pdf
Original checksum: 0df4f90c843c21f78c62c4536928166e
Well formed: true
Valid: true
File author: nimalara
Page count: 152
File language: en-US
Activity of users you follow
User Activity Date