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Permanent link (DOI): https://doi.org/10.7939/R3JW86W3D

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Effect of porcine luteinizing hormone on intrafollicular milieu and gene expression in granulosa cells and oocytes in dairy cows Open Access

Descriptions

Other title
Subject/Keyword
Follicle
Granulosa cells
Dairy cows
Pregnancy rate
Luteinizing hormone
Oocytes
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Behrouzi, Amir
Supervisor and department
Dr. Divakar Ambrose
Examining committee member and department
Dr. John Kastelic
Dr. Michael Dyck
Department
Department of Agricultural, Food, and Nutritional Science
Specialization
Animal Science
Date accepted
2014-01-28T13:49:12Z
Graduation date
2014-06
Degree
Master of Science
Degree level
Master's
Abstract
In previous research, the use of porcine luteinizing hormone (pLH) in lieu of gonadotropin-releasing hormone (GnRH) for synchronizing ovulation in a fixed-timed artificial insemination protocol in dairy cows improved pregnancy rates (42 vs 28%) without increasing progesterone concentrations. The mechanisms associated with this increase in pregnancy rates are not known. We hypothesized that exposure to pLH alters intrafollicular protein/gene expression compared to GnRH treatment, particularly the oocyte-expressed members of the TGF-β superfamily, and EGF-like growth factor, which regulate oocyte competence. To address this, two studies were conducted to determine whether replacing pLH with GnRH to synchronize ovulation in dairy cows altered intrafollicular milieu and gene expression in mural granulosa cells and oocyte cumulus complexes. The results indicated that the relative abundance of BMP-15, GDF-9 and TGF-β1 were greatly increased in pLH-treated cows. Since these factors promote cumulus expansion and oocyte competence, they may have contributed to the pregnancy increase previously reported.
Language
English
DOI
doi:10.7939/R3JW86W3D
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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