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Recombinant expression of plant diacylglycerol acyltransferases from tissues that accumulate saturated fatty acids Open Access


Other title
sea buckthorn pulp, cocoa bean, diacylglycerol acyltransferases, triacylglycerol, cloning
Type of item
Degree grantor
University of Alberta
Author or creator
Zhang, Ying
Supervisor and department
Weselake, Randall J. (Agricultural, Food and Nutritional Science)
Examining committee member and department
Gaenzle, Michael (Agricultural, Food and Nutritional Science)
Weselake, Randall J. (Agricultural, Food and Nutritional Science)
Scarpella, Enrico (Biological Science)
Department of Agricultural, Food, and Nutritional Science
Plant Science
Date accepted
Graduation date
Master of Science
Degree level
Vegetable oils enriched in saturated fatty acids (SFAs) could provide solid-fat functionality and decrease our reliance on hydrogenated oils. This thesis focuses on the characterization of enzymes that can preferentially incorporate SFAs into triacylglycerol (TAG). Acyl-CoA:diacylglycerol acyltransferase (DGAT) catalyzes the final reaction in acyl-CoA-dependent TAG biosynthesis, and may have a substantial effect on the flow of carbon into TAG. DGAT cDNAs encoding type-1 or 2 enzymes were isolated from sea buckthorn (Hippophae rhamnoides) pulp and cocoa (Theobroma cacao) beans, both of which contain oils enriched in SFAs. Expression of the HrDGATs or TcDGATs resulted in different ratios of palmitic to stearic acid in the TAG of yeast mutant strain deficient in TAG synthesis. Furthermore, expression of HrDGAT1 in developing seeds of the Arabidopsis DGAT1 knockout mutant restored TAG accumulation and altered the fatty acid composition. These results suggest that HrDGATs or TcDGATs could potentially be used to increase the SFA content of vegetable oils.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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