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Permanent link (DOI): https://doi.org/10.7939/R3D02B

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Characterization and silencing of differentially abundant proteins from Pyrenophora tritici-repentis Open Access

Descriptions

Other title
Subject/Keyword
virulence
exo-1,3-β-glucanase
tan spot
Pyrenophora tritici-repentis
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Fu, Heting
Supervisor and department
Strelkov, Stephen (Department of Agricultural, Food and Nutritional Science)
Examining committee member and department
Kav, Nat (Department of Agricultural, Food and Nutritional Science)
Deyholos, Michael (Biological sciences)
Department
Department of Agricultural, Food and Nutritional Science
Specialization

Date accepted
2011-08-11T17:47:54Z
Graduation date
2011-11
Degree
Master of Science
Degree level
Master's
Abstract
Tan spot, caused by Pyrenophora tritici-repentis, is an important foliar disease of wheat. Three genes (stp, unp and glu) encoding proteins previously found to be more abundant in pathogenic versus non-pathogenic fungal isolates were cloned, characterized, heterologously expressed, and silenced with the RNA silencing vector pSilent-1. The unp gene encoded a 16.9 kDa protein belonging to a superfamily of glycine-rich RNA-binding proteins, but growth and virulence of an unp-silenced strain of the fungus were not significantly different from the wild-type. The stp gene, which was not significantly silenced, encoded a 15.0 kDa protein homologous to a CipC-like antibiotic response protein. The glu gene encoded an exo-1,3-β-glucanase, 46.7 kDa in mass. Four glu-silenced strains were obtained. The strain in which silencing was strongest exhibited reduced growth, produced fewer appressoria, and caused less disease than the wild-type. The results suggest that exo-1,3-β-glucanase contributes to the development and virulence of P. tritici-repentis.
Language
English
DOI
doi:10.7939/R3D02B
Rights
License granted by Heting Fu (heting1@ualberta.ca) on 2011-08-08T07:05:40Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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