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Permanent link (DOI): https://doi.org/10.7939/R3SM3B

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Characterizing patterns of tissue tropism and environmental reservoirs of PrPCWD in infected cervids Open Access

Descriptions

Other title
Subject/Keyword
Prion
Protein misfolding cyclic amplification
Chronic wasting disease
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Price, Luke M.
Supervisor and department
Belosevic, Miodrag (Biological Sciences)
Neumann, Norman (Public Health Sciences)
Examining committee member and department
Jeon, Byeong Hwa (Public Health Sciences)
Hanington, Patrick (Public Health Sciences)
Aiken, Judd (Agriculture, Food & Nutritional Sciences)
Department
Department of Public Health Sciences
Specialization
Environmental Health Sciences
Date accepted
2013-10-03T09:17:56Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
Chronic wasting disease (CWD) is a fatal prion disease affecting cervids. Believed to be transmitted horizontally, the factors affecting CWD contagion are unknown, but bodily fluids or excretions have been implicated as possible routes of transmission. Protein misfolding cyclic amplification (PMCA) was used as an ultra-sensitive method of detection of prion protein (PrPCWD) across four different cervids: white-tailed deer (WTD), elk, mule deer, and red deer. Strong correlations were observed between traditional diagnostic tests and PMCA for detection of CWD, validating the PMCA PrPCWD assay as a potential diagnostic tool. Assessment of PrPCWD tissue tropism and shedding of CWD prions in the secreta/excreta of infected WTD revealed high levels of PrPCWD in organs other than skeletal muscle throughout the course of infection. PrPCWD was detected in urine, saliva and fecal samples at high levels during the course of infection suggesting that concentrated foci of infectious prions may be sporadically present in the environment.
Language
English
DOI
doi:10.7939/R3SM3B
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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Last modified: 2015:10:12 13:26:18-06:00
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File title: CHAPTER 1: INTRODUCTION AND LITERATURE REVIEW
File author: Mike Belosevic
Page count: 206
File language: en-CA
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