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Prevention of cervical cancer through the characterization of E6 and E7 mRNA transcriptional activity as biological markers of human papillomavirus infections Open Access


Other title
human papillomavirus
cervical cancer
Type of item
Degree grantor
University of Alberta
Author or creator
Tchir, Jayme Dianna Radford
Supervisor and department
Fuller, Jeff (Laboratory Medicine and Pathology)
Examining committee member and department
LaPointe, Paul (Cell Biology)
Tyrrell, Gregory (Laboratory Medicine and Pathology)
Russell, Laurie (Laboratory Medicine and Pathology)
Medical Sciences- Laboratory Medicine and Pathology

Date accepted
Graduation date
Master of Science
Degree level
The quantification of human papillomavirus (HPV) oncogene transcripts, E6 and E7, may be predictive of viral oncogenesis and cancer progression. The main objectives of this study were to determine the HPV genotype prevalence and distribution in Edmonton, Alberta, and characterize a quantifiable association of HPV E6/E7 mRNA expression with the presence of cervical disease. Successful clinical trial design and patient enrolment lead to the first controlled characterization of HPV genotype epidemiology in Alberta. HPV-16 was identified as the most prevalent genotype, followed by several non-vaccine genotypes (HPV-31, -52). Despite rigorous experimentation and a significant correlation between high-risk HPV infection and cervical lesions (p<0.05), absolute quantification of viral oncogenesis was unsuccessful. The ability to quantify oncogene transcriptional activity may, in time, revolutionize cervical cancer screening programmes, akin to the Pap smear several decades ago. However, as experienced in this study and in others, great challenges and even greater questions remain unanswered.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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