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Permanent link (DOI): https://doi.org/10.7939/R3638S

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Development of a Binding-induced DNA Nanosensor Based on Fluorescence Resonance Energy Transfer Open Access

Descriptions

Other title
Subject/Keyword
Binding-induced DNA assembly
Nanosensor
Fluorescence Resonance Energy Transfer
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Li, Xukun
Supervisor and department
Le, X. Chris (Laboratory Medicine and Pathology; Chemistry)
Examining committee member and department
Zhang, Hongquan (Laboratory Medicine and Pathology)
Le, X. Chris (Laboratory Medicine and Pathology; Chemistry)
Campbell, Robert E. (Chemistry)
Department
Department of Chemistry
Specialization

Date accepted
2013-01-31T10:18:33Z
Graduation date
2013-06
Degree
Master of Science
Degree level
Master's
Abstract
This thesis describes a binding-induced DNA nanosensor and its application to homogeneous detection of DNA and proteins. This nanosensor was based on the binding-induced assembly of fluorescently labeled DNA on the DNA-functionalized quantum dots, resulting in fluorescence resonance energy transfer (FRET) between the quantum dots (605 QDs) and the fluorescent dye (Cy5). The sensor was applied to the detection of single-nucleotide polymorphism (SNP) in the p53 gene sequence and the detection of platelet derived growth factor, with pM detection limits. This strategy can be applied to developing assays for a wide range of targets by functionalizing the surface of quantum dots with appropriate affinity ligands (e.g., aptamers and antibodies).
Language
English
DOI
doi:10.7939/R3638S
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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