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Theses and Dissertations

Development of a Binding-Induced DNA Assembly Assay for Carbohydrate Antigen 19-9 Open Access


Other title
real-time PCR
DNA assembly
pancreatic cancer
CA 19-9
carbohydrate antigen 19-9
cancer marker detection
Type of item
Degree grantor
University of Alberta
Author or creator
Wagner, Megan K.
Supervisor and department
Li, Xing-Fang (Laboratory Medicine and Pathology)
Examining committee member and department
Le, X. Chris (Laboratory Medicine and Pathology/Chemistry)
Zhang, Hongquan (Laboratory Medicine and Pathology)
Acker, Jason (Laboratory Medicine and Pathology)
Leslie, Elaine (Physiology/Laboratory Medicine and Pathology)
Medical Sciences- Laboratory Medicine and Pathology

Date accepted
Graduation date
Master of Science
Degree level
Binding-induced DNA assembly (BINDA) was initially developed for protein detection. To demonstrate the homogeneous detection of carbohydrates using this principle, a BINDA assay for the pancreatic cancer marker carbohydrate antigen 19-9 (CA 19-9) was developed. The assay uses two DNA- antibody conjugates as probes, with the DNA oligonucleotides designed to have a complementary base-pairing sequence. Binding to CA 19-9 brings the probes into close proximity, allowing the complementary regions to hybridize. Enzymatic ligation joins the probe oligonucleotides and amplification and detection of the ligation product by real-time PCR is used to quantify CA 19-9. The principle was first demonstrated using streptavidin as a model target before developing and optimizing the BINDA assay for CA 19-9. CA 19-9 could be detected in both buffer and human serum. This study is the first description of the use of BINDA for carbohydrate analysis.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File title: BINDA for CA 19-9
File author: Megan Wagner
Page count: 120
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