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Analysis of the biological effects of Therapeutic ultrasound on orthodontically induced tooth root resorption repair Open Access


Other title
Root resorption
Prevention and repair
Type of item
Degree grantor
University of Alberta
Author or creator
Al-Daghreer, Saleh M
Supervisor and department
Doschak, Michael (Pharmacy & Pharmaceutical Sciences)
El-Bialy, Tarek (Dentistry)
Examining committee member and department
Moussa, Walied (Mechanical Engineering)
Major, Paul (Dentistry)
Sloan, Alastair (Oral Surg, Med & Path, Dental School Cardiff U.)
Tsui, Ying (Electrical Engineering)
Heo, Giseon (Dentistry)
Tanaka, Eiji (Dept of Ortho & Dentofacial Orthopedics, U Tokushima Grad School)
Medical Sciences-Dentistry
Date accepted
Graduation date
Doctor of Philosophy
Degree level
The aim of this thesis was to investigate the biological effects of LIPUS on orthodontically induced inflammatory resorption (OIIRR) in in vivo and in vitro models. The in vitro samples were obtained from healthy human premolars and cultured for 5-days or 24-hours with application of LIPUS at different doses. The in vivo, we tested 10 beagle dogs where orthodontic movement was carried out for four weeks with a continuous force of 1 N/side; using a split mouth model. After 4-weeks, mandibles were resected into blocks involving the fourth premolar and its periodontal tissue. The 4th premolars were evaluated using micro-CT, histologically and immunohistochemically. In both models, LIPUS was generated with repetition rate of 1 KHz. Each pulse has a square envelop with duration of 200 microseconds and a carrier frequency of 1.5 MHz and 30mW/cm2 intensity. Daily application of LIPUS on TSOC for 5-days did not have any effect on the predentin thickness layer, and had an adverse effect on the odontoblast cell count. A one-time application of LIPUS in a 5-day culture of TSOC increased the predentin thickness in all groups. Also it increased the odontoblast cell count in the 5, 10 and 15 minutes application groups. LIPUS application for 10 minutes upregulated the expression of collagen-I and DMP-1 in the short term (24 h), where 5-minutes application upregulated the expression of collagen-I only. LIPUS did not affect the rate of orthodontic tooth movement and had a trend of increasing it with increased population of the osteoclasts attached to the alveolar bone in the PDL. LIPUS significantly reduced the number of OIIRR initiation areas by 71%, reduced its total volume by 68%, and reduced its volume relative to the affected root total volume by 70%. LIPUS induced the formation of precemntum layer, thicker cementum and reparative cellular cementum.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Long term effect of Low Intensity Pulsed Ultrasound on a human tooth slice organ culture. Al-Daghreer S, Doschak M, Sloan AJ, Major PW, Heo G, Scurtescu C, Tsui YY, El-Bialy T. Arch Oral Biol. 2012 Jun;57(6):760-8. Epub 2011 Dec 3.

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