Study of Non-Covalent Protein-Carbohydrate Interactions by Nanoelectrospray Ionization Mass Spectrometry and Surface Plasmon Resonance Open Access
- Other title
- Type of item
- Degree grantor
University of Alberta
- Author or creator
- Supervisor and department
Klassen, John (Chemistry)
- Examining committee member and department
Peterson, Nils (Chemistry)
Li, Liang (Chemistry)
Department of Chemistry
- Date accepted
- Graduation date
Master of Science
- Degree level
This thesis describes the application of electrospray ionization mass spectrometry (ESI-MS) techniques to identify and quantify antibody-antigen interactions in vitro. Also potential application of nanodiscs (ND) as membrane model in ESI-MS and surface plasmon resonance (SPR) spectroscopy has been investigated.
A direct nanoelectrospray mass spectrometry assay (nanoESI-MS) was developed for quantification of specific binding between a monoclonal antibody (mAb) and its cognate antigen. A qualitative investigation was performed on cross-reactivity of antibodies. NanoESI-MS was also employed to investigate the effects of fatty acid chain lengths on its degree of incorporation into ND. The results can be utilized for synthesis purposes where biologically important carbohydrates require a proper carbon chain for incorporation into ND.
ND technology was also employed to investigate protein-ligand interaction using SPR spectroscopy. The results open up further investigation on the effect of the position of ganglioside binding sites incorporated into ND and ability of their binding to the receptors in SPR.
- Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
- Citation for previous publication
Aneika C. Leney, Reza Rezaei Darestani, Jun Li, Sanaz Nikjah, Elena N. Kitova, Chunxia Zou, Christopher W. Cairo, Zi Jian Xiong, Gilbert G. Privé, and John S. Klassen. Picodiscs for Facile Protein-Glycolipid Interaction Analysis
Analytical Chemistry 2015 87 (8), 4402-4408
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