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Permanent link (DOI): https://doi.org/10.7939/R3CF9JF8G

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Bacteriophages as antimicrobial agents against bacterial contaminants in yeast fermentation processes Open Access

Descriptions

Other title
Subject/Keyword
Ethanol
Phages
Yeast fermentation
Contaminants
Biofuel
Antimicrobials
Bacteriophages
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Bertozzi Silva, Juliano
Supervisor and department
Sauvageau, Dominic (Chemical and Materials Engineering)
Examining committee member and department
Gaenzle, Michael (Agricultural, Food & Nutritional Science)
Semagina, Natalia (Chemical and Materials Engineering)
Sauvageau, Dominic (Chemical and Materials Engineering)
Department
Department of Chemical and Materials Engineering
Specialization
Chemical Engineering
Date accepted
2013-09-27T20:48:40Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
Bacterial contaminants are ubiquitous in yeast fermentation processes for biofuel production. In general, they compete with yeasts for nutrients, reducing overall production yield. The present project investigates the application of bacteriophages (phages), viruses infecting and killing prokaryotes, as potential antibacterial agents in yeast fermentation processes. A phage cocktail was applied to control contamination while preventing the development of bacterial resistance. The study identifies and quantifies the effects of parameters influencing infection/contamination. The data obtained were ultimately used to develop a mathematical model detailing the dynamics of the populations involved (yeasts, bacteria and phages). The results showed that the addition of phages cocktail at relatively low initial multiplicity of infection was sufficient to reduce contamination and allowed the yields of yeast and ethanol to reach values equivalent to those of axenic yeast cultures. Moreover, the model showed good fit to the experimental results.
Language
English
DOI
doi:10.7939/R3CF9JF8G
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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