Download the full-sized PDF
This file is in the following communities:
|Faculty of Graduate Studies and Research|
This file is not currently in any collections.
Antiviral and cytokine responses of human mast cells to influenza A virus infection Open Access
- Other title
- Type of item
- Degree grantor
University of Alberta
- Author or creator
- Supervisor and department
Befus, A. Dean (Medicine)
- Examining committee member and department
Barry, Michele (Medical Microbiology and Immunology)
Vethanayagam, Dilini (Medicine)
Adamko, Darryl (Pediatrics)
Marshall, Jean S. (Microbiology and Immunology, Dalhousie University)
Magor, Katharine E. (Biological Sciences)
Befus, A. Dean (Medicine)
Department of Medicine
- Date accepted
- Graduation date
Doctor of Philosophy
- Degree level
Mast cells are immune cells important in innate immunity. Besides their role in asthma and allergies, mast cells are critical effector cells against various pathogens. Mast cells are established to be protective against bacterial infections, but little is known about their functions in viral infections.
Mast cells are abundant in the lungs where influenza A virus (FluA) enter the host. We measured mRNA transcription, protein translation, and synthesis of new viral particles in FluA-treated mast cells. While expression of FluA mRNA and proteins followed similar time courses in both mast cells and epithelial cells, mast cells released a near absence of FluA.
We also studied mast cell cytokine release in response to FluA and other viral-associated stimuli such as TLR ligands and type I interferons. Mast cells released the cytokines IL-6 and TGF-, and chemokines CXCL8 and CCL5 in response to various viral stimuli. However, different stimuli were capable of inducing release of different mediator subsets, demonstrating the specificity of mast cell responses.
Since FluA infection of mast cells produce little new FluA virions, we investigated whether FluA induces expression of antiviral proteins in mast cells. FluA treatment resulted in mast cell expression of antiviral proteins, namely myxovirus resistance protein A, protein kinase R, interferon stimulated gene 15, viral stress inducible protein 56, and endothelial nitric oxide synthase.
Next, we performed co-culture experiments using FluA-infected epithelial cells with or without the addition of mast cells. Our results showed that mast cells in co-culture inhibited the expression of the viral hemagglutinin protein in FluA-infected epithelial cells. Also, preliminary results showed that addition of mast cells protected epithelial cells from FluA infection by limiting the release of FluA particles and reducing epithelial cell death.
Our discovery that mast cells produce little virus and express antiviral proteins suggest that mast cells have antiviral mechanisms to restrict FluA infection. This concept was further supported by evidence that mast cells are protective against FluA infection in epithelial cells. This research provides a better understanding of mast cells in innate immunity and may reveal unique antiviral mechanisms valuable in the development of antiviral therapeutics.
- License granted by Candy Marcet (email@example.com) on 2010-08-12T03:41:59Z (GMT): Permission is hereby granted to the University of
Alberta Libraries to reproduce single copies of
this thesis and to lend or sell such copies for
private, scholarly or scientific research purposes
only. Where the thesis is converted to, or
otherwise made available in digital form, the
University of Alberta will advise potential users
of the thesis of the above terms.
The author reserves all other publication and
other rights in association with the copyright in
the thesis, and except as herein provided, neither
the thesis nor any substantial portion thereof may
be printed or otherwise reproduced in any material
form whatsoever without the author's prior written
- Citation for previous publication
- Date Uploaded
- Date Modified
- Audit Status
- Audits have not yet been run on this file.
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 2842565
Last modified: 2015:10:12 11:21:33-06:00
Filename: Marcet_Candy_Fall 2010.pdf
Original checksum: 06e27e76e7399ca5966b0701c2e1c2c1
Well formed: false
Status message: Invalid page tree node offset=930446
Status message: Unexpected error in findFonts java.lang.ClassCastException: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject cannot be cast to edu.harvard.hul.ois.jhove.module.pdf.PdfDictionary offset=2835337
Page count: 100