Fibre-Type Specific Nuclear Localisation Patterns of NFAT-isoforms in CLFS Induced Skeletal Muscle Adaptations

• Author / Creator

  McDonald, Pamela C

• The purpose of this investigation was to characterize the nuclear localisation patterns of NFAT-c1, -c2, -c3, and -c4 in relation to chronic low-frequency stimulation induced fibre type transitions. A model has been suggested to describe the roles of all 4 NFAT isoforms in activity-dependent skeletal muscle fibre transformation. The findings of the present study are consistent with different yet related roles for the NFAT isoforms in fast-to-slow fibre type transformation. NFAT-c1 nuclear expression was associated with transition toward slower fibre types (type-I, type-IIA, type-IID(X)), whereas NFAT-c3 nuclear expression was associated with transformations surrounding the type-IIA fibres. The nuclear expression patterns of NFAT-c2 and NFAT-c4 are consistent with their involvement in fibre maintenance and cell survival.

• Subjects / Keywords

  • Skeletal muscles
  • Nuclear factor of activated T-cells
  • Immunohistochemistry
  • Myosin heavy chain

• Graduation date

  Spring 2014

• Type of Item

  Thesis

• Degree

  Master of Science

• DOI

  https://doi.org/10.7939/R37659N5N

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.