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Permanent link (DOI): https://doi.org/10.7939/R39P9Z

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MUC1 is a novel costimulatory and coinhibitory molecule of human T cells Open Access

Descriptions

Other title
Subject/Keyword
costimulation
NF-AT
T cell
coinhibition
MUC1
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Konowalchuk, Jeffrey
Supervisor and department
Agrawal, Babita (Surgery)
Examining committee member and department
Anderson, Colin (Surgery)
Rayat, Gina (Surgery)
Suresh, Mavanur (Pharmacy)
Department
Department of Surgery
Specialization

Date accepted
2009-07-07T15:50:49Z
Graduation date
2009-11
Degree
Master of Science
Degree level
Master's
Abstract
MUC1, a protein of epithelial and carcinoma cells, has recently been shown on activated T cells where it inhibits CD3-stimulated proliferation. Two immunoregulatory domains similar to ITAM and ITIMs are present on its cytoplasmic tail, suggesting that MUC1 can act as both a costimulatory and coinhibitory molecule of T cells. In my work, I have examined immunoregulatory function of MUC1 on human T cells. We first showed that MUC1, when ligated in a population of unpurified T cells with an anti-CD3 and a crosslinking antibody, enhances proliferative and cytokine responses in a NF-AT-dependent manner by recruiting the AP-1 transcription factor and translocating it into the nucleus. With purified CD3+ T cells, we instead observed inhibition after MUC1/CD3 coligation and crosslinking. Reconstituting with irradiated CD3- cells, we discovered that MUC1 costimulation is dependent on the amount of accessory cells. These data imply a novel role for MUC1 in T cell immunoregulation.
Language
English
DOI
doi:10.7939/R39P9Z
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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