ERA

Download the full-sized PDF of Single Chain Fraction Variable Binding Molecules as Bone-Targeting Therapeutics and DiagnosticsDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3FH1F

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

Single Chain Fraction Variable Binding Molecules as Bone-Targeting Therapeutics and Diagnostics Open Access

Descriptions

Other title
Subject/Keyword
scFv
CTX
osteocalcin
osteoporosis
RANK
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Lam, Michael
Supervisor and department
Doschak, Michael (Pharmacy and Pharmaceutical Sciences)
Examining committee member and department
Dixon, Walter (Agric, Food & Nutritional Science)
Kaur, Kamaljit (Pharmacy and Pharmaceutical Sciences)
Jurasz, Paul (Pharmacy and Pharmaceutical Sciences)
El-Kadi, Ayman (Pharmacy and Pharmaceutical Sciences)
Department
Faculty of Pharmacy and Pharmaceutical Sciences
Specialization

Date accepted
2011-08-28T15:18:21Z
Graduation date
2011-11
Degree
Master of Science
Degree level
Master's
Abstract
Osteoporosis is a disease characterized by lowering of bone mass and subsequent bone fracture. Although successful antiresorptive treatment options are commercially available, they have disadvantages such as poor bioavailability and significant side effects. Using phage display, an in vitro high throughput screening method, we sought to generate single chain fraction variable (scFv) against osteoclast surface receptors and bone turnover markers, for the evaluation of their potential as drug-targeted delivery platforms for improved bioavailability of current therapeutics and as immunodiagnostic assay reagents in osteoporosis. With our current in vitro result, it can be concluded that scFv, although having weaker binding affinity than IgG antibody, still possesses good selective binding against antigens. With this method of generating scFv being more cost-effective and less labour intensive, scFv reagents can become a viable option in site-directed drug delivery and immunodiagnostic for osteoporosis and possibly for cross application to other bone-modifying disease such as osteoarthritis.
Language
English
DOI
doi:10.7939/R3FH1F
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2014-05-01T17:31:05.088+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 913122
Last modified: 2015:10:12 10:19:23-06:00
Filename: Lam_Michael_Fall 2011.pdf
Original checksum: 86dff1ece28f23432f4eb4b08bd70d9d
Well formed: true
Valid: true
File title: -
File author: Michael
Page count: 122
File language: en-US
Activity of users you follow
User Activity Date