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Permanent link (DOI): https://doi.org/10.7939/R3M32NJ7N

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Tripartite-motif family members in the White Pekin duck (Anas platyrhynchos) modulate antiviral gene expression Open Access

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Other title
Subject/Keyword
TRIMs
innate immune modulation
Anas platyrhynchos
Tripartite-motif family
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Blaine, Alysson H.
Supervisor and department
Magor, Katharine E. (Biological Sciences)
Examining committee member and department
Smiley, James (Medical Microbiology and Immunology)
Belosevic, Miodrag (Biological Sciences)
Stafford, James (Biological Sciences)
Department
Department of Biological Sciences
Specialization
Physiology, Cell & Developmental Biology
Date accepted
2013-09-27T20:47:27Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
Wild waterfowl, including mallard ducks, are the natural reservoir of avian influenza A virus and are resistant to highly pathogenic strains. This is primarily due to the robust innate immune response of ducks. Shortly after exposure to both highly pathogenic (A/Viet Nam/1203/04 (H5N1)) and low pathogenic (A/mallard/BC/500/05 (H5N2)) avian influenza, many immune genes are upregulated including members of the diverse tripartite-motif (TRIM) family. TRIM proteins have species-specific antiviral roles in a variety of viral infections. I have identified a contig of TRIM genes located adjacent to the MHC locus in the White Pekin duck (Anas platyrhynchos) genome. A duplication of the TRIM27.1 gene (TRIM27.1a and TRIM27.1b) has occurred. Using quantitative real-time PCR (qPCR) I determined that both TRIM27.1a and TRIM27.1b are upregulated 34- and 5-fold, respectively, at 1 day post-infection with VN1203. In a co-transfection experiment I determined that TRIM27.1a and TRIM27.1b have opposite effects on expression, decreasing and increasing transcription of antiviral genes, respectively.
Language
English
DOI
doi:10.7939/R3M32NJ7N
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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