ERA

Download the full-sized PDF of Gene expression profiles during the initial phase of salt stress in riceDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3P26Q49V

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Biological Sciences, Department of

Collections

This file is in the following collections:

Journal Articles (Biological Sciences)

Gene expression profiles during the initial phase of salt stress in rice Open Access

Descriptions

Author or creator
Kawasaki, S.
Borchert, C.
Deyholos, M.
Wang, H.
Brazille, S.
Kawai, K.
Galbraith, D.
Bohnert, H. J.
Additional contributors
Subject/Keyword
Oryza-Sativa-L
Abscisic-Acid
Salinity Resistance
Arabidopsis-Thaliana
Plants
Oxidative Stress
Beta-Glucosidase
Active Oxygen
Freezing Tolerance
Gluta Thione S-Transferases
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Transcript regulation in response to high salinity was investigated for salt-tolerant rice (var Pokkali) with microarrays including 1728 cDNAs from libraries of salt-stressed roots. NaCl at 150 mM reduced photosynthesis to one tenth of the prestress value within minutes. Hybridizations of RNA to microarray slides probed for changes in transcripts from 15 min to 1 week after salt shock. Beginning 15 min after the shock, Pokkali showed upregulation of transcripts. Approximately 10% of the transcripts in Pokkali were significantly upregulated or downregulated within 1 hr of salt stress. The initial differences between control and stressed plants continued for hours but became less pronounced as the plants adapted over time. The interpretation of an adaptive process was supported by the similar analysis of salinity-sensitive rice (var IR29), in which the immediate response exhibited by Pokkali was delayed and later resulted in downregulation of transcription and death. The upregulated functions observed with Pokkali at different time points during stress adaptation changed over time. Increased protein synthesis and protein turnover were observed at early time points, followed by the induction of known stress-responsive transcripts within hours, and the induction of transcripts for defenserelated functions later. After 1 week, the nature of upregulated transcripts (e.g., aquaporins) indicated recovery.
Date created
2001
DOI
doi:10.7939/R3P26Q49V
License information
Rights
© 2001 American Society of Plant Biologists. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.
Citation for previous publication
Kawasaki, S., Borchert, C., Deyholos, M., Wang, H., Brazille, S., Kawai, K., Galbraith, D., & Bohnert, H. J. (2001). Gene expression profiles during the initial phase of salt stress in rice. The Plant Cell, 13(4), 889-906. doi: http:/​/​dx.​doi.​org/​10.​1105/​tpc.​13.​4.​889.
Source
Link to related item

File Details

Date Uploaded
Date Modified
2014-04-29T19:14:49.213+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 342167
Last modified: 2015:10:12 15:27:18-06:00
Filename: PC_13_2001_889.pdf
Original checksum: 4c95099ee8be50d873310015534950b6
Well formed: true
Valid: false
Status message: Invalid page tree node offset=669
File title: 00-0448
File author: comp
Activity of users you follow
User Activity Date