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Permanent link (DOI): https://doi.org/10.7939/R3CJ87X6Q

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Broadening of genetic diversity in spring canola (Brassica napus L.) by use of yellow sarson and Canadian spring Brassica rapa L. Open Access

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Other title
Subject/Keyword
SSR marker
genetic diversity
canola
Brassica napus
breeding
Broadening
interspecific cross
Brassica rapa
backcross
pedigree breeding
oilseed rape
GSL
glucosinolates
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Attri, Rohit
Supervisor and department
Basu, Urmila (Department of Agricultural, Food & Nutritional Science)
Rahman, Habibur (Department of Agricultural, Food & Nutritional Science)
Examining committee member and department
Hall, Jocelyn (Department of Biological Science)
Basu, Urmila (Department of Agricultural, Food & Nutritional Science)
Rahman, Habibur (Department of Agricultural, Food & Nutritional Science)
Department
Department of Agricultural, Food, and Nutritional Science
Specialization
Plant Science
Date accepted
2015-05-22T09:58:18Z
Graduation date
2015-11
Degree
Master of Science
Degree level
Master's
Abstract
Canada is the top producer of Brassica oilseeds [B. napus L. (n = 19, AC genome)] in the world. Genetic diversity has declined in this crop in the recent years due to use of only superior and genetically narrow gene pool in breeding. Presence of adequate genetic diversity is important for further improvement of this crop through breeding. Genetically distinct germplasm of B. napus or its allied species can be used to broaden genetic diversity in Canadian B. napus canola. However, limited efforts have been made to utilize genetic diversity of the progenitor species B. rapa (n = 10, A genome) and B. oleracea (n = 9, C genome) in the breeding of this crop as interspecific cross often introduces undesirable traits in the breeding program. This M.Sc. thesis research was undertaken to develop genetically distinct B. napus lines through interspecific crosses between B. napus canola and B. rapa. For this, three genetically distinct B. rapa lines were used. The F1’s of B. napus × B. rapa interspecific crosses were self-pollinated for F2 as well as backcrossed to the B. napus parent for BC1F1 progenies. Pedigree breeding was applied where selection for plant fertility and glucosinolate content was done in each generation. SSR marker analysis of the F4 plants revealed that the three populations derived from B. napus × B. rapa crosses are genetically distinct from each other as well as from the B. napus parent; thus, the advanced generation populations derived from the progeny of these plants expected to carry allelic diversity of the B. rapa parents. Plant fertility and glucosinolates content in many of the F7 and BC1F4 families reached close to the B. napus parent. Flow cytometric analysis of F6 and BC1F3 families for nuclear DNA content indicated that many families are euploid B. napus type. Findings from this thesis research suggest that genetically distinct, fertile, euploid B. napus canola lines can be developed from both F2 and BC1F1 of the B. napus × B. rapa interspecific crosses.
Language
English
DOI
doi:10.7939/R3CJ87X6Q
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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