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Permanent link (DOI): https://doi.org/10.7939/R3J96097M

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Optimization of a two-step desolvation method for preparing gelatin nanoparticles and cell uptake studies in 143B osteosarcoma cancer cells Open Access

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Author or creator
Azarmi, S.
Huang, Y.
Chen, H.
McQuarrie, S.
Abrams, D.
Roa, W.
Finlay, W. H.
Miller, G. G.
Löbenberg, R.
Additional contributors
Subject/Keyword
Degradation
Glutaralhehyde
Nanospheres
Sustained-release
Biodegradable
Albumin microspheres
In-vitro
Cross-linking
Particle-size
Drug-delivery system
Microparticles
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Abstract: PURPOSE: To establish a matrix of parameters to synthesize nanoparticles of different sizes and to investigate the cellular uptake of these nanoparticles by osteosarcoma cancer cells in order to investigate their potential as therapeutic drug-delivery carriers. METHODS: Gelatin A and B were used to synthesize nanoparticles by a two-step desolvation process. Different parameters were investigated, including temperature, pH, concentration of glutaraldehyde, type of desolvating agent and nature of gelatin. For cell uptake studies, Texas Red labeled nanoparticles were incubated with 143B osteosarcoma cells and then evaluated using confocal laser scanning microscopy (CLSM). RESULTS: The systematic investigation of the synthesis parameters showed that it is possible to prepare gelatin-based nanoparticles with different particle sizes and a narrow size distribution. Temperature and nature of the gelatin were the most important synthesis factors. Bioimaging using CLSM showed uptake of the nanoparticles by 143B osteosarcoma cancer cells. CONCLUSIONS: Osteosarcoma cancer cells take up gelatin nanoparticles. This might improve the clinical effectiveness of anti-cancer treatments if nanoparticles are used as a drug delivery system and has important implications for future cancer treatment strategies.
Date created
2006
DOI
doi:10.7939/R3J96097M
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This work is made available under the terms of the Creative Commons Attribution - Share Alike 3.0 Unported license, http://creativecommons.org/licenses/by-sa/3.0/.
Citation for previous publication
Shirzad Azarmi, Yuan Huang, Hua Chen, Steve McQuarrie, Douglas Abrams, Wilson Roa, Warren H Finlay, Gerald G Miller, Raimar Löbenberg. (2006). Optimization of a two-step desolvation method for preparing gelatin nanoparticles and cell uptake studies in 143B osteosarcoma cancer cells. J Pharm Pharm Sci, 9(1), 124-132.
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