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Permanent link (DOI): https://doi.org/10.7939/R31C1TS31

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Intestinal Uptake of Barley Protein Nanoparticles as Delivery Vehicles for Bioactive Compounds Open Access

Descriptions

Other title
Subject/Keyword
Barley protein
Nanoparticles
Caco-2 cells
Uptake
Bioavailability
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Zhou, Ying
Supervisor and department
Chen, Lingyun (Agricultural, Food and Nutritional Science)
Examining committee member and department
Field, Catherine (Agricultural, Food and Nutritional Science)
Vine, Donna (Agricultural, Food and Nutritional Science)
Guan, Leluo (Agricultural, Food and Nutritional Science)
Department
Department of Agricultural, Food, and Nutritional Science
Specialization
Food Science and Technology
Date accepted
2013-08-30T09:09:11Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
The use of nanoparticles as nutrient delivery vehicles enables the enhancement of the oral bioavailability and health promoting benefits of bioactive compounds. Barley protein nanoparticles were developed in previous study for hydrophobic compound delivery. The objective of the present study was to evaluate the cytotoxicity of the nanoparticles and to characterize their intestinal uptake properties using in vitro and ex vivo models. The nanoparticles showed low cytotoxicity in Caco-2 cells. Their cellular uptake was dependent on time, concentration and temperature, suggesting transcytosis pathway. Significantly greater β-carotene uptake (15%) was observed in Caco-2 cells when delivered by nanoparticles compared to control (2.6%). The nanoparticles also showed adhesion and permeation abilities in rat jejunum tissues. Findings from this study demonstrated the uptake improving effect of barley protein nanoparticles and suggested their potential as nutrient delivery vehicles for the development of novel functional foods.
Language
English
DOI
doi:10.7939/R31C1TS31
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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