ERA

Download the full-sized PDF of An examination of linking and blocking procedures for use in deflection cantilever array-based protein detectionDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3V701

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

An examination of linking and blocking procedures for use in deflection cantilever array-based protein detection Open Access

Descriptions

Other title
Subject/Keyword
MEMS
linking
array
cantilever
ELISA
biosensor
protein
blocking
detection
fluorescence
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
van den Hurk, Remko
Supervisor and department
Dr. Stephane Evoy
Examining committee member and department
Dr. Christopher Backhouse (Electrical and Computer Engineering)
Dr. Thomas Thundat (Chemical and Materials Engineering)
Department
Department of Electrical and Computer Engineering
Specialization

Date accepted
2011-09-26T23:55:40Z
Graduation date
2011-11
Degree
Master of Science
Degree level
Master's
Abstract
In this project common linking and blocking procedures were examined for use in multiplexed deflection cantilever array-based detection of proteins. A human interferon gamma (INF-γ) enzyme-linked immunosorbent assay (ELISA) kit was used to evaluate the effectiveness of the linking and blocking procedures using fluorescence and ELISA assays. The most effective linking and blocking procedures were then implemented on arrays of deflection cantilevers. Separating the active and reference cantilever signals proved to be challenging due to varying deflection in buffer solution. Background subtractions were implemented which reduced the nonspecific buffer deflection. Following the background subtractions 16 out of 52 experiments produced one or more data sets with a clearly defined separation between all the active and reference signals. The most successful linkers, reference cantilever blockers and cantilever backside blockers were Prolinker B, glutaradehyde and EDC/Sulfo-NHS; canine capture antibody and INF-γ; and a thermal PEG-silanization procedure and PEG-thiol, respectively.
Language
English
DOI
doi:10.7939/R3V701
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2014-05-01T01:39:46.331+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 5541626
Last modified: 2015:10:12 14:13:41-06:00
Filename: van den Hurk_Remko_Fall 2011.pdf
Original checksum: 1f2c8bfea6491918081ee3775129b779
Well formed: false
Valid: false
Status message: Invalid page tree node offset=971110
Status message: Unexpected error in findFonts java.lang.ClassCastException: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject cannot be cast to edu.harvard.hul.ois.jhove.module.pdf.PdfDictionary offset=3045
Status message: Invalid Annotation list offset=5493794
Status message: Outlines contain recursive references.
File title: Abstract
Activity of users you follow
User Activity Date