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Permanent link (DOI): https://doi.org/10.7939/R3K931F4J

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Synthesis, Characterization and Biological Studies of the Phospholipid Coated Gold Nanoparticles (GNPs) Open Access

Descriptions

Other title
Subject/Keyword
uptake
phospholipid
Gold nanoparticles
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Wang, Meijing
Supervisor and department
Dr. Nils O. Petersen, Department of Chemistry
Examining committee member and department
Dr. Mark McDermott, Department of Chemistry
Dr. Christopher Cairo, Department of Chemistry
Department
Department of Chemistry
Specialization

Date accepted
2013-08-28T13:48:41Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
Gold nanoparticles (GNPs) have been applied as diagnostic and therapeutic agents because they can be targeted, localized, and be heated to cause cell death. However, their use has been limited by their relatively low biocompatibility. In this work, we coated the GNPs surface by a biocompatible phospholipid bilayer composed of 1-stearoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (SOPG). In order to use the fluorescence, a small amount of the NBD labeled PG (NBD-PG) was introduced to the phospholipid bilayer. We synthesized and characterized the NBD labeled SOPG coated GNPs (NBD-SOPG-GNPs) and gold nanorods with different size and shape. We tested the interaction of 30 nm NBD-SOPG-GNPs with three different cell lines (A549, C2C12 and C2bbe1) to investigate their uptake and intracellular fate as well as the response of the cells to the presence of the NBD-SOPG-GNPs. Our results demonstrated that NBD-SOPG-GNPs has different uptake destinations in three cell lines.
Language
English
DOI
doi:10.7939/R3K931F4J
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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