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Permanent link (DOI): https://doi.org/10.7939/R3109K

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Investigation on the uptake of functional proteins and infectious prions into wheat plants through the root system Open Access

Descriptions

Other title
Subject/Keyword
protoplast
prion
disease transmission
chronic wasting disease
root morphology
fluorescent protein
food safety
protein uptake
nitrogen nutrition
wheat
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Rasmussen, Jay D
Supervisor and department
McAllister, Tim A (Agricultural, Food and Nutritional Science)
Kav, Nat NV (Agricultural, Food and Nutritional Science)
Examining committee member and department
Guan, Leluo (Agricultural, Food and Nutritional Science)
Neumann, Norman F (School of Public Health)
McAllister, Tim A (Agricultural, Food and Nutritional Science)
McKenzie, Debbie (Biology)
Kav, Nat NV (Agricultural, Food and Nutritional Science)
Department
Department of Agricultural, Food, and Nutritional Science
Specialization
Animal Science
Date accepted
2013-11-13T09:51:11Z
Graduation date
2014-06
Degree
Master of Science
Degree level
Master's
Abstract
Prions are the proteinaceous particle responsible for infections in a class of neurodegenerative diseases. These diseases affect a number of mammals including cervids where it is termed Chronic Wasting Disease (CWD). Prions enter the environment and persist for years. Plants have the ability to take up large organic molecules like proteins and bacteria as a potential nitrogen source. This project used wheat (Triticum aestivum L.) to determine the reason for protein uptake in plants and if prions are also taken up by plants. We found that bovine serum albumin was not a suitable nitrogen source for plants but uptake of ovalbumin into the stem was possible when minor root damage was present. Conversely, CWD prions bound to the outside of wheat roots and were not taken up into the stem. This work suggests that plants do not act as a vector in the transmission of prion diseases such as CWD.
Language
English
DOI
doi:10.7939/R3109K
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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