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Permanent link (DOI): https://doi.org/10.7939/R36Q1SR4N

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Methane Production in Oil Sands Tailings under Nitrogen-Depleted Conditions Open Access

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Other title
Subject/Keyword
Bioavailable
Methane
Methanogenesis
Mining
15N2
Mature fine tailings
N2
Bacteria
Nutrients
Acetylene reduction
Microorganisms
Deficient
Tailings ponds
Anaerobic
Albian
Remediation
Metabolism
Archaea
Reclamation
Biogenic
Citrate
Methanogens
Mothur
Pyrosequecing
Microbial communities
Nitrogen
Stable isotope
MFT
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Collins, Catherine Elizabeth Victoria
Supervisor and department
Siddique, Tariq (Renewable Resources)
Examining committee member and department
Ulrich, Ania (Cival and Environmental Engineering)
Foght, Julia (Biological Sciences)
Department
Department of Renewable Resources
Specialization
Land Reclamation and Remediation
Date accepted
2013-07-04T13:38:32Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
Surface mining for bitumen extraction results in production of tailings that are deposited into large ponds. Tailings in the ponds support diverse microbial communities capable of metabolizing organic compounds and producing biogenic gases (methane, CH4 and carbon dioxide, CO2). Because of low endogenous concentrations of bioavailable nitrogen (N), tailings ponds might be deficient in available N (NO3-/NH4+). This study examined the potential of N2-fixation mediated methanogenic degradation of citrate and petroleum hydrocarbons in oil sands tailings under N-depleted conditions. Anaerobic primary cultures were set up with available N or N-deficient medium under N2 headspace using mature fine tailings (MFT) and amended with citrate as a carbon source. Citrate was metabolized to CH4 under both N available and deficient conditions suggesting N2-fixation mediated degradation of citrate. Acetylene reduction assay and incorporation of 15N2 stable isotope into microbial biomass supported N2-fixation during citrate metabolism.
Language
English
DOI
doi:10.7939/R36Q1SR4N
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File author: Victoria
Page count: 141
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