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Permanent link (DOI): https://doi.org/10.7939/R3W67N

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CELLULASE IMMOBILIZATIONS WITH HIGHLY RETAINED ENZYMATIC ACTIVITIES Open Access

Descriptions

Other title
Subject/Keyword
Biorefinery
Cellulases
Immobilization
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Ikeda, Yuko
Supervisor and department
Bressler, David (Agricultural, Food, and Nutritional Science)
Examining committee member and department
McMullen, Lynn (Agricultural, Food, and Nutritional Science)
Sauvageau, Dominic (Chemical & Materials Engineering)
Saville, Bradley (Chemical Engineering and Applied Chemistry)
McCaffrey, Wiliam (Chemical & Materials Engineering)
Bressler, David (Agricultural, Food, and Nutritional Science)
Department
Department of Agricultural, Food, and Nutritional Science
Specialization
Bioresource and Food Engineering
Date accepted
2013-09-26T07:01:36Z
Graduation date
2013-11
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
Immobilization has the potential to make enzyme utilization more cost efficient, but reduced enzymatic activity makes it unfeasible. The purpose of this study was to develop immobilized cellulase systems on silica supports that retain high enzymatic activity. Two of the four systems created resulted in better-than-expected productivity during hydrolysis and make good candidates for further research. The immobilized systems on fumed non-porous silica retained enzyme activity equivalent to free cellulases when hydrolyzing crystalline cellulose. However, sugar composition analysis indicated that immobilization decreased cellobiose conversion to glucose, resulting in substantial cellobiose production. More research is required to understand the mechanism at work. The immobilized cellulases obtained wider pH stability, but displayed decreased thermal and ionic stability. Storage stability was unchanged. One major finding was increased enzyme reusability, with activity remaining at 30% after nine uses. When lignocellulose biomass was used for hydrolysis, production was substantially lower than when using free cellulases. It is hypothesized that the immobilized cellulases had difficulty hydrolyzing the amorphous regions of the lignocellulose. Although further optimization is necessary, the immobilized cellulase systems developed in this study contribute greatly to increasing the commercial viability of hydrolysis and lignocellulose pretreatment.
Language
English
DOI
doi:10.7939/R3W67N
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File author: Yuko Ikeda
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